Characterization of local pH changes in brain using fast-scan cyclic voltammetry with carbon microelectrodes.
Characterization of local pH changes in brain using fast-scan cyclic voltammetry with carbon microelectrodes.
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DOI：
10.1021/ac102399n
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发表时间：
2010-12-01
影响因子：
7.4
通讯作者：
Wightman, R. Mark
中科院分区：
文献类型：
--
作者：
Takmakov, Pavel;Zachek, Matthew K.;Keithley, Richard B.;Bucher, Elizabeth S.;McCarty, Gregory S.;Wightman, R. Mark
大脑中短暂的局部pH值变化是神经活动的重要标志，可用于跟踪作为行为、学习和记忆的生物学基础的代谢过程。有几种方法可以测量pH值波动与足够的时间分辨率在自由移动的动物。以前，在碳纤维微电极上的快速扫描循环伏安法用于测量这种pH瞬变。然而，在体内记录的pH值变化的循环伏安图中的电位依赖性电流的起源尚不清楚。目前的工作探讨了这些峰的性质，并确定了其中一些峰的起源。鉴定了与pH CV的电容性质相关的峰。电化学惰性物质（如芳香胺和钙）的吸附可抑制该峰，并且是体内和体外数据不一致的原因。此外，我们确定了一个额外的峰在体内pH CV有关的存在下，3，4-二羟基乙酸（DOPAC）在脑细胞外液。为了评估碳纤维传感器的体内性能，通过麻醉大鼠吸入二氧化碳来诱导由高碳酸血症诱导的脑酸中毒。事实证明，高碳酸血症是诱导体内pH值强烈变化的有用工具，可以确认用FSCV观察到的pH信号。
Transient local pH changes in the brain are important markers of neural activity that can be used to follow metabolic processes that underlie the biological basis of behavior, learning and memory. There are few methods that can measure pH fluctuations with sufficient time resolution in freely moving animals. Previously, fast-scan cyclic voltammetry at carbon-fiber microelectrodes was used for the measurement of such pH transients. However, the origin of the potential dependent current in the cyclic voltammograms for pH changes recorded in vivo was unclear. The current work explored the nature of these peaks and established the origin for some of them. A peak relating to the capacitive nature of the pH CV was identified. Adsorption of electrochemically inert species, such as aromatic amines and calcium could suppress this peak, and is the origin for inconsistencies regarding in vivo and in vitro data. Also, we identified an extra peak in the in vivo pH CV relating to the presence of 3,4-dihydroxyacetic acid (DOPAC) in the brain extracellular fluid. To evaluate the in vivo performance of the carbon-fiber sensor, carbon dioxide inhalation by an anesthetized rat was used to induce brain acidosis induced by hypercapnia. Hypercapnia is demonstrated to be a useful tool to induce robust in vivo pH changes, allowing confirmation of the pH signal observed with FSCV.
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影响因子：
4.2
作者：
Heien, MLAV;Phillips, PEM;Wightman, RM
通讯作者：
Wightman, RM
影响因子：
7.4
作者：
Bath, BD;Michael, DJ;Wightman, RM
通讯作者：
Wightman, RM
影响因子：
3
作者：
Davidson, C;Ellinwood, EH;Lee, TH
通讯作者：
Lee, TH
影响因子：
7.4
作者：
ELLIOTT, CM;MURRAY, RW
通讯作者：
MURRAY, RW
影响因子：
48
作者：
Clark, Jeremy J.;Sandberg, Stefan G.;Wanat, Matthew J.;Gan, Jerylin O.;Horne, Eric A.;Hart, Andrew S.;Akers, Christina A.;Parker, Jones G.;Willuhn, Ingo;Martinez, Vicente;Evans, Scott B.;Stella, Nephi;Phillips, Paul E. M.
通讯作者：
Phillips, Paul E. M.