Nicotinamide riboside supplementation confers marginal metabolic benefits in obese mice without remodeling the muscle acetyl-proteome.
Nicotinamide riboside supplementation confers marginal metabolic benefits in obese mice without remodeling the muscle acetyl-proteome.
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DOI：
10.1016/j.isci.2021.103635
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发表时间：
2022-01-21
期刊：
影响因子：
5.8
通讯作者：
Muoio DM
中科院分区：
文献类型：
--
作者：
Williams AS;Koves TR;Pettway YD;Draper JA;Slentz DH;Grimsrud PA;Ilkayeva OR;Muoio DM
烟酰胺核苷补充剂（NRS）已被吹捧为通过增强烟酰胺腺嘌呤二核苷酸（NAD+）生物合成、线粒体功能和/或NAD依赖性沉默调节蛋白脱乙酰酶的活性来促进心脏代谢和肌肉骨骼健康的营养药物。本研究使用C57 BL/6 NJ小鼠，在饮食诱导的肥胖背景下，考察了NRS对全身能量稳态、骨骼肌线粒体功能以及乙酰赖氨酸蛋白质组相应变化的影响。该研究还包括一个转基因小鼠模型，该模型对sirtuin通量和相关的NAD+消耗，特别是在肌肉组织中，有更大的需求。一般而言，在慢性高脂饮食的中点给药时，NRS可略微改善全身葡萄糖控制，但在饮食开始后作为预防性治疗时，NRS则无改善。与预期结果相反，该研究几乎没有证据表明NRS增加组织NAD+水平，增强线粒体功能和/或减轻饮食诱导的骨骼肌蛋白质组的过度乙酰化。膳食NR补充剂（NRS）提高肥胖小鼠的血浆和肌肉NAM水平NRS给予肥胖后略微改善的葡萄糖控制和线粒体功能NRS不对抗肥胖诱导的肌肉乙酰蛋白质组重构NRS在体重增加期间不保护葡萄糖控制和线粒体功能生理学;蛋白质组学;营养学
Nicotinamide riboside supplements (NRS) have been touted as a nutraceutical that promotes cardiometabolic and musculoskeletal health by enhancing nicotinamide adenine dinucleotide (NAD+) biosynthesis, mitochondrial function, and/or the activities of NAD-dependent sirtuin deacetylase enzymes. This investigation examined the impact of NRS on whole body energy homeostasis, skeletal muscle mitochondrial function, and corresponding shifts in the acetyl-lysine proteome, in the context of diet-induced obesity using C57BL/6NJ mice. The study also included a genetically modified mouse model that imposes greater demand on sirtuin flux and associated NAD+ consumption, specifically within muscle tissues. In general, whole body glucose control was marginally improved by NRS when administered at the midpoint of a chronic high-fat diet, but not when given as a preventative therapy upon initiation of the diet. Contrary to anticipated outcomes, the study produced little evidence that NRS increases tissue NAD+ levels, augments mitochondrial function, and/or mitigates diet-induced hyperacetylation of the skeletal muscle proteome. Dietary NR supplementation (NRS) raises plasma and muscle NAM levels in obese mice NRS given post obesity slightly improved glucose control and mitochondrial function NRS did not oppose obesity-induced remodeling of the muscle acetyl-proteome NRS during weight gain did not protect glucose control and mitochondrial function Physiology; Proteomics; Nutrition
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影响因子：
16
作者：
Hirschey MD;Shimazu T;Jing E;Grueter CA;Collins AM;Aouizerat B;Stančáková A;Goetzman E;Lam MM;Schwer B;Stevens RD;Muehlbauer MJ;Kakar S;Bass NM;Kuusisto J;Laakso M;Alt FW;Newgard CB;Farese RV Jr;Kahn CR;Verdin E
通讯作者：
Verdin E
影响因子：
29
作者：
Frederick DW;Loro E;Liu L;Davila A Jr;Chellappa K;Silverman IM;Quinn WJ 3rd;Gosai SJ;Tichy ED;Davis JG;Mourkioti F;Gregory BD;Dellinger RW;Redpath P;Migaud ME;Nakamaru-Ogiso E;Rabinowitz JD;Khurana TS;Baur JA
通讯作者：
Baur JA
影响因子：
14.9
作者：
Deutsch EW;Csordas A;Sun Z;Jarnuczak A;Perez-Riverol Y;Ternent T;Campbell DS;Bernal-Llinares M;Okuda S;Kawano S;Moritz RL;Carver JJ;Wang M;Ishihama Y;Bandeira N;Hermjakob H;Vizcaíno JA
通讯作者：
Vizcaíno JA
影响因子：
2.9
作者：
Glancy, Brian;Willis, Wayne T.;Balaban, Robert S.
通讯作者：
Balaban, Robert S.
影响因子：
6.1
作者：
Crisol, Barbara M.;Veiga, Camilla B.;Ropelle, Eduardo R.
通讯作者：
Ropelle, Eduardo R.