Aluminium-induced ion transport in Arabidopsis: the relationship between Al tolerance and root ion flux.
Aluminium-induced ion transport in Arabidopsis: the relationship between Al tolerance and root ion flux.
复制标题
DOI：
10.1093/jxb/erq143
复制
发表时间：
2010-06
影响因子：
6.9
通讯作者：
Rengel Z
中科院分区：
文献类型：
--
作者：
Bose J;Babourina O;Shabala S;Rengel Z
铝 (Al) 的根毒性与低 pH 值一致；然而，尚不清楚植物对这两个因素的耐受性是否是由相同的机制控制的。为了解决这个问题，我们对铝抗性alr104突变体、两种铝敏感突变体（als3和als5）和野生型拟南芥进行了长期暴露（溶液培养）和短期暴露实验（H+和K+通量、根际pH和质膜电位，Em）的比较。基于生物量积累，als5和alr104表现出对低pH的耐受性，而alr104则耐受低pH/Al联合处理。 als5 和 als3 突变体对铝胁迫的敏感性相似。 Al 敏感突变体（als3 和 als5）中 Al 诱导的远端延伸区 (DEZ) H+ 流入减少和 Al 诱导的成熟区 (MZ) H+ 流出量高于野生型和 alr104 突变体。在低 pH/Al 联合处理下，alr104 和野生型在整个 30 分钟测量期间质膜去极化，而在 Al 敏感突变体（als3 和 als5）中，初始去极化至 –60mV 左右，20 分钟后变为超极化至 –110mV。在 DEZ，Em 的变化对应于 K+ 通量的变化：alr104 和野生型中的 K+ 流出量高于 als3 和 als5 突变体。总之，alr104 突变体中的铝耐受性与 Em 去极化、更高的 K+ 流出和更高的 H+ 流入相关，从而导致在低 pH/铝联合胁迫下根际更加碱性。低 pH 耐受性 (als5) 与低 pH 胁迫下较高的 H+ 吸收有关，但暴露于 Al 会消除这种情况。
Aluminium (Al) rhizotoxicity coincides with low pH; however, it is unclear whether plant tolerance to these two factors is controlled by the same mechanism. To address this question, the Al-resistant alr104 mutant, two Al-sensitive mutants (als3 and als5), and wild-type Arabidopsis thaliana were compared in long-term exposure (solution culture) and in short-term exposure experiments (H+ and K+ fluxes, rhizosphere pH, and plasma membrane potential, Em). Based on biomass accumulation, als5 and alr104 showed tolerance to low pH, whereas alr104 was tolerant to the combined low-pH/Al treatment. The sensitivity of the als5 and als3 mutants to the Al stress was similar. The Al-induced decrease in H+ influx at the distal elongation zone (DEZ) and Al-induced H+ efflux at the mature zone (MZ) were higher in the Al-sensitive mutants (als3 and als5) than in the wild type and the alr104 mutant. Under combined low-pH/Al treatment, alr104 and the wild type had depolarized plasma membranes for the entire 30 min measurement period, whereas in the Al-sensitive mutants (als3 and als5), initial depolarization to around –60 mV became hyperpolarization at –110 mV after 20 min. At the DEZ, the Em changes corresponded to the changes in K+ flux: K+ efflux was higher in alr104 and the wild type than in the als3 and als5 mutants. In conclusion, Al tolerance in the alr104 mutant correlated with Em depolarization, higher K+ efflux, and higher H+ influx, which led to a more alkaline rhizosphere under the combined low-pH/Al stress. Low-pH tolerance (als5) was linked to higher H+ uptake under low-pH stress, which was abolished by Al exposure.
登录
查看更多内容
影响因子：
6.9
作者：
Koyama, H;Toda, T;Hara, T
通讯作者：
Hara, T
影响因子：
4.9
作者：
Cuin, TA;Shabala, S
通讯作者：
Shabala, S
DOI：
10.1073/pnas.0700117104
发表时间：
2007-06-05
影响因子：
11.1
作者：
Iuchi, Satoshi;Koyama, Hiroyuki;Kobayashi, Masatomo
通讯作者：
Kobayashi, Masatomo
影响因子：
7.4
作者：
KASAMO, K
通讯作者：
KASAMO, K
影响因子：
9.4
作者：
Calba, H;Jaillard, B
通讯作者：
Jaillard, B