Comparison of three typing methods for clinical and environmental isolates of Aspergillus fumigatus
Comparison of three typing methods for clinical and environmental isolates of Aspergillus fumigatus
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烟曲霉临床和环境分离株三种分型方法的比较
DOI：
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发表时间：
1995
影响因子：
9.4
通讯作者：
B. Lasker
中科院分区：
文献类型：
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作者：
Diming Lin;P. Lehmann;B. Hamory;A. Padhye;E. Durry;R. Pinner;B. Lasker
为了评估用于曲霉病暴发流行病学分析的程序，我们使用三种分型方法对 35 株烟曲霉分离株进行了分析：同工酶分析 (IEA)、随机扩增多态性 DNA (RAPD) 分析和限制性内切酶分析 (REA)。 21 个分离株来自同一家医院，其中 4 个分离株来自不同患者。 3 个临床分离株来自不同的医院，11 个临床或环境分离株来自培养物保藏中心。通过 IEA，碱性磷酸酶、酯酶和过氧化氢酶的模式可区分九种类型。相比之下，使用 5 种不同的 RAPD 引物获得了 22 种类型，其中 3 种（R108、R151 和 UBC90）可以检测到 21 种类型。对用 XbaI、XhoI 或 SalI 消化的基因组 DNA 进行限制性内切核酸酶分析，分别检测到 3、17 和 13 种不同的 REA 类型，并通过结合 XhoI 和 SalI REA 的数据鉴定出 22 种类型。通过 REA、IEA 和 RAPD 模式的组合可以获得 28 种类型。总体而言，结果表明分离株之间存在显着的遗传变异。尽管两个分离株具有明显不同的基因型，但它们的形态特征和外抗原与烟曲霉一致。该分析将有助于规划曲霉病的流行病学研究。
To evaluate procedures used for epidemiologic analysis of outbreaks of aspergillosis, we analyzed a collection of 35 Aspergillus fumigatus isolates using three typing methods: isoenzyme analysis (IEA), random amplified polymorphic DNA (RAPD) analysis, and restriction endonuclease analysis (REA). Twenty-one isolates were from a single hospital, with four isolates coming from different patients. Three clinical isolates came from a different hospital, and 11 clinical or environmental isolates were derived from a culture collection. With IEA, the patterns of alkaline phosphatase, esterase, and catalase discriminated nine types. In contrast, 22 types were obtained with five different RAPD primers, and 21 types could be detected with three of these (R108, R151, and UBC90). Restriction endonuclease analysis of genomic DNA, digested with either XbaI, XhoI, or SalI, detected 3, 17, and 13 different REA types, respectively, and 22 types were identified by combining the data from the XhoI and SalI REAs. Twenty-eight types were obtainable with a combination of REA, IEA, and RAPD patterns. Overall, the results pointed to substantial genetic variation among the isolates. Though two isolates had markedly distinct genotypes, their morphologic features and exoantigens were consistent with their being A. fumigatus. The analysis will help in planning epidemiologic studies of aspergillosis.
RAPD（任意引物）PCR 在区分相关细菌菌株方面比多位点酶电泳更灵敏。
影响因子：
14.9
作者：
Wang,G;Whittam,TS;Berg,CM;Berg,DE
通讯作者：
Berg,DE