RAPD (arbitrary primer) PCR is more sensitive than multilocus enzyme electrophoresis for distinguishing related bacterial strains.
RAPD (arbitrary primer) PCR is more sensitive than multilocus enzyme electrophoresis for distinguishing related bacterial strains.
复制标题
RAPD（任意引物）PCR 在区分相关细菌菌株方面比多位点酶电泳更灵敏。
DOI：
10.1093/nar/21.25.5930
复制
发表时间：
1993
影响因子：
14.9
通讯作者：
Berg,DE
中科院分区：
文献类型：
--
作者：
Wang,G;Whittam,TS;Berg,CM;Berg,DE
RAPD（随机扩增多态性DNA）指纹识别方法，利用任意序列的单个引物进行低强度PCR扩增，生成菌株特异性的匿名DNA片段阵列，相对于广泛使用的基于蛋白质的多位点酶电泳（MLEE）分型方法进行了校准。对全世界引起腹泻病的大肠杆菌15个主要类群各5株（共75株）进行了RAPD指纹图谱分析。每一组由20种诊断酶标记物的MLEE分型无法区分的分离株组成。在我们的RAPD测试中，每个MLEE组中的3个或更多不同的亚群都被5个引物区分开来，当5个引物获得的数据合并时，75个分离株中有74个被区分出来。因此，RAPD分型比MLEE分型在物种亲缘系间的区分要灵敏得多。尽管它们的敏感性不同，但从MLEE和RAPD数据推断菌株之间的一般关系相同。因此，我们的研究结果推荐使用RAPD方法研究细菌群体的遗传结构和进化，以及流行病学。
The RAPD (random amplified polymorphic DNA) fingerprinting method, which utilizes low stringency PCR amplification with single primers of arbitrary sequence to generate strain-specific arrays of anonymous DNA fragments, was calibrated relative to the widely used, protein-based multilocus enzyme electrophoretic (MLEE) typing method. RAPD fingerprinting was carried out on five isolates from each of 15 major groups ofEscherichia colistrains that cause diarrheal disease worldwide (75 isolates in all). Each group consisted of isolates that were not distinguishable from one another by MLEE typing using 20 diagnostic enzyme markers. In our RAPD tests, three or more distinct subgroups in each MLEE group were distinguished with each of five primers, and 74 of the 75 isolates were distinguished when data obtained with five primers were combined. Thus, RAPD typing is far more sensitive than MLEE typing for discriminating among related strains of a species. Despite their different sensitivities, the same general relationships among strains were inferred from MLEE and RAPD data. Thus, our results recommend use of the RAPD method for studies of bacterial population genetic structure and evolution, as well as for epidemiology.
登录
查看更多内容
影响因子：
64.5
作者：
M. Donnenberg
通讯作者：
M. Donnenberg
影响因子：
3.1
作者：
WHITTAM, TS;WOLFE, ML;WILSON, RA
通讯作者：
WILSON, RA
影响因子：
14.9
作者：
AKOPYANZ, N;BUKANOV, NO;BERG, DE
通讯作者：
BERG, DE
六种寄生原生动物的遗传特征：随机引物 DNA 分型和多位点酶电泳之间的奇偶性。
DOI：
10.1073/pnas.90.4.1335
发表时间：
1993
影响因子：
11.1
作者：
Tibayrenc,M;Neubauer,K;Barnabé,C;Guerrini,F;Skarecky,D;Ayala,FJ
通讯作者：
Ayala,FJ
英国分离的产生 Vero 细胞毒素的大肠杆菌 O 157 的噬菌体分型：1989-91
影响因子：
4.2
作者：
J. Frost;T. Cheasty;A. Thomas;B. Rowe
通讯作者：
B. Rowe