SEC14 phospholipid transfer protein is involved in lipid signaling-mediated plant immune responses in Nicotiana benthamiana.

SEC14 phospholipid transfer protein is involved in lipid signaling-mediated plant immune responses in Nicotiana benthamiana.
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DOI:
10.1371/journal.pone.0098150
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发表时间:
2014
期刊:
影响因子:
3.7
通讯作者:
Hikichi Y
Hikichi Y
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Kiba A;Galis I;Hojo Y;Ohnishi K;Yoshioka H;Hikichi Y

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我们之前鉴定了与 SEC14 基因磷脂转移蛋白超家族相关的基因,该基因在本塞姆氏烟草 (NbSEC14) 中响应青枯菌感染而被诱导。我们在这里报道 NbSEC14 通过磷脂周转在植物免疫反应中发挥作用。 NbSEC14 沉默会损害防御相关 PR-4 的表达以及茉莉酸 (JA) 及其衍生物 JA-Ile 的积累。 NbSEC14 的瞬时表达诱导 PR-4 基因表达。在 NbSEC14 沉默的植物中,二酰基甘油激酶、磷脂酶 C 和 D 的活性以及无毒力的青枯菌引起的二酰基甘油和磷脂酸的合成均降低。 NbSEC14 的瞬时表达增强了信号脂质的积累以及二酰基甘油激酶和磷脂酶的激活。这些结果表明 NbSEC14 蛋白在脂质信号传导代谢和植物先天免疫反应之间的界面中发挥作用。
We previously identified a gene related to the SEC14-gene phospholipid transfer protein superfamily that is induced in Nicotiana benthamiana (NbSEC14) in response to infection with Ralstonia solanacearum. We here report that NbSEC14 plays a role in plant immune responses via phospholipid-turnover. NbSEC14-silencing compromised expression of defense–related PR-4 and accumulation of jasmonic acid (JA) and its derivative JA-Ile. Transient expression of NbSEC14 induced PR-4 gene expression. Activities of diacylglycerol kinase, phospholipase C and D, and the synthesis of diacylglycerol and phosphatidic acid elicited by avirulent R. solanacearum were reduced in NbSEC14-silenced plants. Accumulation of signaling lipids and activation of diacylglycerol kinase and phospholipases were enhanced by transient expression of NbSEC14. These results suggest that the NbSEC14 protein plays a role at the interface between lipid signaling-metabolism and plant innate immune responses.
DOI: 10.1038/347561a0
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