Protein degradation in cultured cells. II. The uptake of chloroquine by rat fibroblasts and the inhibition of cellular protein degradation and cathepsin B1.
Protein degradation in cultured cells. II. The uptake of chloroquine by rat fibroblasts and the inhibition of cellular protein degradation and cathepsin B1.
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培养细胞中的蛋白质降解。 ii。大鼠成纤维细胞对氯喹的摄取以及细胞蛋白降解和组织蛋白酶B1的抑制作用。
DOI：
10.1083/jcb.63.2.430
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发表时间：
1974-11
影响因子：
7.8
通讯作者：
POOLE, B
中科院分区：
文献类型：
--
作者：
WIBO, M;POOLE, B
低浓度的氯喹或中性红可抑制成纤维细胞蛋白质的降解，无论是快速的还是缓慢的周转率。氟可进一步抑制氯喹抑制的细胞。氯喹被成纤维细胞摄取，细胞中的浓度达到培养基中的数百倍。等密度分馏研究表明，在细胞内的氯喹集中在溶酶体，这些含氯喹的溶酶体有一个较低的平衡密度比未处理的细胞的溶酶体。在溶酶体内达到的浓度下，氯喹抑制组织蛋白酶B1，但不抑制组织蛋白酶D。它的结论是，氯喹损害细胞蛋白质的分解后，这些已经进入溶酶体系统，可能通过抑制组织蛋白酶B1。
The degradation of cellular proteins in fibroblasts, both those of rapid and those of slow turnover rates, was inhibited by low concentrations of chloroquine or neutral red in the medium. Cells inhibited by chloroquine can be inhibited further by fluoride. Chloroquine was taken up by the fibroblasts and the concentration in the cells reached several hundred times that in the medium. Isopycnic fractionation studies showed that within the cells the chloroquine was concentrated in the lysosomes, and that these chloroquine-containing lysosomes had a lower equilibrium density than the lysosomes of untreated cells. Chloroquine, at concentrations attained inside the lysosomes, inhibited cathepsin B1 but not cathepsin D. It is concluded that chloroquine impairs the breakdown of cellular proteins after these have entered the lysosome system, probably through inhibition of cathepsin B1.
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影响因子：
7.8
作者：
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影响因子：
7.8
作者：
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通讯作者：
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影响因子：
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作者：
LIE, SO;SCHOFIELD, B
通讯作者：
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