Overexpression of l-galactono-1, 4-lactone dehydrogenase (GLDH) in Lanzhou lily (Lilium davidii var. unicolor) via particle bombardment-mediated transformation
Overexpression of l-galactono-1, 4-lactone dehydrogenase (GLDH) in Lanzhou lily (Lilium davidii var. unicolor) via particle bombardment-mediated transformation
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通过粒子轰击介导的转化在兰州百合（Lilium davidii var. unicolor）中过表达l-半乳糖-1, 4-内酯脱氢酶（GLDH）
DOI：
10.1007/s11627-011-9383-2
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发表时间：
2012-02
期刊：
影响因子：
--
通讯作者：
Shang, Zengzhen
中科院分区：
文献类型：
--
作者：
Shi, Shouguo;Ma, Fengwang;Li, Yonghong;Feng, Fengjuan;Shang, Zengzhen
在中国，兰州百合(Lilium davidii var.)单色)是一种可食用的百合，是抗坏血酸的重要来源。然而，对于这种重要的作物，还没有开发出有效的转化系统。在这里，我们描述了第一次成功地通过基因枪介导百合品种的遗传转化。以苹果L-半乳糖苷-1，4-内酯脱氢酶基因、潮霉素磷酸转移酶基因和含有内含子的β-葡萄糖苷酸酶基因为选择标记。通过GUS分析和聚合酶链式反应，共筛选出35个转基因株系，其中8个经Southern分析得到证实。转基因植株被转移到土壤中，并在温室条件下生长。转基因植株的还原型抗坏血酸和总抗坏血酸(还原型抗坏血酸、 + 氧化型抗坏血酸)水平是非转基因对照的2~7倍。
In China, the native Lanzhou lily (Lilium davidii var. unicolor) is used as an edible lily and is an important source of ascorbic acid. However, an efficient transformation system has not been developed for this important crop. Here, we describe the first successful genetic transformation mediated by particle bombardment in this lily variety. We used pCAMBIA-GLDH, which contains the l-galactono-1, 4-lactone dehydrogenase gene from apple, a hygromycin phosphotransferase gene as a selectable marker, and an intron-containing β-glucuronidase gene as a reporter. In all, 35 transgenic lines were selected via GUS assays and PCR; from these, eight were confirmed by Southern analyses. Transgenic plants were transferred to soil and grown under greenhouse conditions. The levels of reduced ascorbate and total ascorbate (reduced ascorbate + oxidized ascorbate) in transgenic plants were found to be two to seven times those in non-transgenic controls.
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影响因子：
4.3
作者：
L. Tian;F. A. Canli;Xinhua Wang;S. Sibbald
通讯作者：
L. Tian;F. A. Canli;Xinhua Wang;S. Sibbald
影响因子：
6.2
作者：
A. Watad;Dae-Jin Yun;Tracie K. Matsumoto;X. Niu;Y. Wu;A. Kononowicz;R. Bressan;P. Hasegawa
通讯作者：
A. Watad;Dae-Jin Yun;Tracie K. Matsumoto;X. Niu;Y. Wu;A. Kononowicz;R. Bressan;P. Hasegawa
DOI：
--
发表时间：
--
期刊：
Comput. Hum. Behav.
影响因子：
--
作者：
通讯作者：
--
影响因子：
6.5
作者：
de Pinto, MC;Tommasi, F;De Gara, L
通讯作者：
De Gara, L
影响因子：
6.4
作者：
J. J. Chinoy-J.;K. Nanda;O. P. Garg
通讯作者：
J. J. Chinoy-J.;K. Nanda;O. P. Garg