Effects of feeding increasing proportions of corn grain on concentration of lipopolysaccharide in the rumen fluid and the subsequent alterations in immune responses in goats.
Effects of feeding increasing proportions of corn grain on concentration of lipopolysaccharide in the rumen fluid and the subsequent alterations in immune responses in goats.
复制标题
增加玉米粒饲喂比例对山羊瘤胃液中脂多糖浓度及随后免疫反应变化的影响
DOI：
10.5713/ajas.2013.13143
复制
发表时间：
2013-10
期刊：
影响因子：
--
通讯作者：
Mao S
中科院分区：
文献类型：
--
作者：
Huo W;Zhu W;Mao S
本研究旨在探讨饲粮比例增加对山羊瘤胃液中脂多糖（LPS）浓度的影响，以及由此引起的免疫反应的变化，并通过血清淀粉样蛋白A（SAA）和结合珠蛋白（Hp）的浓度反映这一变化。采用3 ×3拉丁方试验设计，将9只山羊随机分为3组，分别饲喂0%、25%和50%玉米粒的日粮。结果表明，随着日粮玉米比例的增加，瘤胃pH值降低（p< 0.001），丙酸（p<0.001）、丁酸（p <0.001）、乳酸（p = 0.013）和总挥发性脂肪酸（p = 0.031）浓度升高，瘤胃内脂多糖水平升高（p<0.001）。随着日粮玉米比例的增加，SAA的浓度增加（p = 0.013）。在饲喂25%和50%玉米的个体山羊的血液中可检测到LPS。实时荧光定量PCR分析表明，随着日粮玉米添加量的增加，拟杆菌门的拷贝数显著降低（p<0.001）（4.61× 109 copies/mL ~ 1.48× 109 copies/mL），相关性分析表明，拟杆菌门的拷贝数与瘤胃内脂多糖水平呈显著负相关。总的来说，这些结果表明，饲喂山羊高比例（50%）的玉米颗粒降低了瘤胃pH值，增加了瘤胃液中的LPS，并倾向于刺激炎症反应。
This study was conducted to investigate the effects of feeding increasing proportions of corn grain on concentration of lipopolysaccharide (LPS) in the rumen fluid and the subsequent alterations in immune responses as reflected by plasma concentrations of serum amyloid A (SAA) and haptoglobin (Hp) in goats. Nine goats were assigned to three diets (0%, 25%, and 50% corn grain) in a 3 ×3 Latin square experimental design. The results showed that as the proportion of dietary corn increased, the ruminal pH decreased (p< 0.001), and the concentrations of propionate (p<0.001), butyrate (p<0.001), lactic acid (p = 0.013) and total volatile fatty acid (p = 0.031) elevated and the ruminal LPS level increased (p<0.001). As the proportion of dietary corn increased, the concentration of SAA increased (p = 0.013). LPS was detectable in the blood of individual goats fed 25% and 50% corn. A real-time PCR analysis showed that the copy number of phylum Bacteroidetes (p<0.001) was reduced (4.61×109copies/mL to 1.48×109copies/mL) by the increasing dietary corn, and a correlation analysis revealed a significant negative correlation between the number of Bacteroidetes and rumen LPS levels. Collectively, these results indicated that feeding goats high proportions (50%) of corn grain decreased the ruminal pH, increased LPS in the rumen fluid and tended to stimulate an inflammatory response.
登录
查看更多内容
影响因子：
3.5
作者：
Ametaj, B. N.;Emmanuel, D. G. V.;Dunn, S. M.
通讯作者：
Dunn, S. M.
影响因子：
4.2
作者：
Denman, Stuart E.;McSweeney, Christopher S.
通讯作者：
McSweeney, Christopher S.
影响因子：
4.4
作者：
Fernando, S. C.;Purvis, H. T., II;DeSilva, U.
通讯作者：
DeSilva, U.
影响因子：
1.5
作者：
Gonzalez, Felix H. D.;Tecles, Fernando;Ceron, Jose J.
通讯作者：
Ceron, Jose J.
影响因子：
3.5
作者：
Emmanuel, D. G. V.;Dunn, S. M.;Ametaj, B. N.
通讯作者：
Ametaj, B. N.