Critical Involvement of Environmental Carbon Dioxide Fixation to Drive Wax Ester Fermentation in Euglena.
Critical Involvement of Environmental Carbon Dioxide Fixation to Drive Wax Ester Fermentation in Euglena.
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DOI：
10.1371/journal.pone.0162827
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Ohta D
中科院分区：
文献类型：
--
作者：
Padermshoke A;Ogawa T;Nishio K;Nakazawa M;Nakamoto M;Okazawa A;Kanaya S;Arita M;Ohta D
研究了多种环境条件下眼虫中蜡酯的积累情况。在黑暗的缺氧条件下，总蜡酯的积累量最高，并且 C28（肉豆蔻醇肉豆蔻酸酯，C14:0-C14:0）在所有研究条件中普遍存在。光照缺氧条件下蜡酯的产生几乎完全受到抑制，而供给外源无机碳源可以恢复蜡酯发酵，表明蜡酯发酵需要外源碳源。 13C标记实验揭示了蜡酯衍生的奇数脂肪酸中的特定同位素富集，表明外源提供的CO2通过丙酰辅酶A途径通过逆三羧酸（TCA）循环掺入蜡酯中。添加磷酸烯醇丙酮酸羧激酶 (PEPCK) 抑制剂 3-巯基甲酸显着影响 13C 掺入作为奇数脂肪酸生物合成中间体的柠檬酸盐和苹果酸盐中，表明 PEPCK 反应参与驱动蜡酯发酵。此外，琥珀酸的 13C 富集模式表明，除了 PEPCK 反应之外，CO2 同化可能还通过其他途径进行。目前的结果表明缺氧CO2同化机制是加强裸藻蜡酯发酵的重要目标。
Accumulation profiles of wax esters in Euglena gracilis Z were studied under several environmental conditions. The highest amount of total wax esters accumulated under hypoxia in the dark, and C28 (myristyl-myristate, C14:0-C14:0) was prevalent among all conditions investigated. The wax ester production was almost completely suppressed under anoxia in the light, and supplying exogenous inorganic carbon sources restored wax ester fermentation, indicating the need for external carbon sources for the wax ester fermentation. 13C-labeling experiments revealed specific isotopic enrichment in the odd-numbered fatty acids derived from wax esters, indicating that the exogenously-supplied CO2 was incorporated into wax esters via the propionyl-CoA pathway through the reverse tricarboxylic acid (TCA) cycle. The addition of 3-mercaptopicolinic acid, a phosphoenolpyruvate carboxykinase (PEPCK) inhibitor, significantly affected the incorporation of 13C into citrate and malate as the biosynthetic intermediates of the odd-numbered fatty acids, suggesting the involvement of PEPCK reaction to drive wax ester fermentation. Additionally, the 13C-enrichment pattern of succinate suggested that the CO2 assimilation might proceed through alternative pathways in addition to the PEPCK reaction. The current results indicate that the mechanisms of anoxic CO2 assimilation are an important target to reinforce wax ester fermentation in Euglena.
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影响因子：
3.5
作者：
INUI, H;MIYATAKE, K;KITAOKA, S
通讯作者：
KITAOKA, S
DOI：
10.1016/0304-4165(81)90251-8
发表时间：
1981-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
PEAK, JG;PEAK, MJ
通讯作者：
PEAK, MJ
影响因子：
7.4
作者：
PONSGENSCHMIDT, E;SCHNEIDER, T;BETZ, A
通讯作者：
BETZ, A
影响因子：
4.3
作者：
CODD, GA;MERRETT, MJ
通讯作者：
MERRETT, MJ
影响因子：
3.5
作者：
Ferreira, Veronica dos Santos;Rocchetta, Iara;Levin, Mariano J.
通讯作者：
Levin, Mariano J.