Rice RBH1 Encoding A Pectate Lyase is Critical for Apical Panicle Development.
Rice RBH1 Encoding A Pectate Lyase is Critical for Apical Panicle Development.
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编码果胶酸裂解酶的水稻 RBH1 对于顶端圆锥花序的发育至关重要
DOI：
10.3390/plants10020271
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发表时间：
2021-01-30
期刊：
影响因子：
--
通讯作者：
Wu C
中科院分区：
文献类型：
--
作者：
He D;Liang R;Long T;Yang Y;Wu C
穗部形态是决定水稻产量的主要因素之一。穗败育是一种典型的穗形态缺陷，由于小穗发育不良而导致产量下降。为了进一步阐明水稻穗部败育的分子机制，对一个在穗部发育过程中表现出严重顶端小穗败育的转移DNA（T-DNA）插入突变体rbh 1进行了鉴定和特征分析。rbh 1 -1突变体在退化小穗中表现出明显的细胞形态和结构改变。分子遗传学研究表明RBH 1编码一种果胶酸裂解酶蛋白。以多聚半乳糖醛酸（PGA）为底物，对水稻穗秃头1号（RBH 1）蛋白质进行果胶酸裂解酶比活力测定，结果表明该酶对PGA具有较强的降解能力。此外，免疫组织化学分析表明，果胶的降解在rbh 1 -1突变体中受到抑制。进一步的分析表明，rbh 1 -1退化小穗中活性氧（ROS）水平显著增加。综上所述，我们的研究结果表明RBH 1是必要的穗形成和防止穗败育。
Panicle morphology is one of the main determinants of the rice yield. Panicle abortion, a typical panicle morphological defect results in yield reduction due to defective spikelet development. To further elucidate the molecular mechanism of panicle abortion in rice, a rice panicle bald head 1 (rbh1) mutant with transfer DNA (T-DNA) insertion showing severely aborted apical spikelets during panicle development was identified and characterized. The rbh1-1 mutant showed obviously altered cell morphology and structure in the degenerated spikelet. Molecular genetic studies revealed that RBH1 encodes a pectate lyase protein. Pectate lyase-specific activity of Rice panicle Bald Head 1 (RBH1) protein assay using polygalacturonic acid (PGA) as substrates illustrated that the enzyme retained a significant capacity to degrade PGA. In addition, immunohistochemical analysis showed that the degradation of pectin is inhibited in the rbh1-1 mutant. Further analysis revealed that a significant increase in reactive oxygen species (ROS) level was found in degenerated rbh1-1 spikelets. Taken together, our findings suggest that RBH1 is required for the formation of panicle and for preventing panicle abortion.
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