Effects of acupuncture on the miR-146a-mediated IRAK1/TRAF6/NF-κB signaling pathway in rats with sarcopenia induced by D-galactose.
Effects of acupuncture on the miR-146a-mediated IRAK1/TRAF6/NF-κB signaling pathway in rats with sarcopenia induced by D-galactose.
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DOI：
10.21037/atm-22-6082
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发表时间：
2023-01-31
影响因子：
--
通讯作者：
Chen, Shaoqing
中科院分区：
文献类型：
--
作者：
Jin, Jing;Yang, Zhengyu;Liu, Haichao;Guo, Mingling;Chen, Borui;Zhu, Haoming;Wang, Yu;Lin, Jianping;Wang, Shizhong;Chen, Shaoqing
衰老过程中的肌肉减少症与无菌、低度慢性炎症密切相关。将50只SD大鼠随机分为5组，分别为对照组、D-半乳糖（D-gal）组、D-半乳糖+针刺（DA）组、D-D-半乳糖+非穴位注射（DN）组和D-半乳糖氨基酸合剂（DAA）组。用D-半乳糖12周建立衰老大鼠模型。对照组大鼠每日给予0.9%生理盐水。治疗组采用针刺或氨基酸混合液干预，每日1次，连续4周。采用苏木精-伊红染色（H&E）、透射电镜（TEM）和末端脱氧核苷酸转移酶缺口末端标记（TUNEL）等方法观察针刺的作用。采用免疫荧光、Western blotting、实时荧光定量PCR和酶联免疫吸附试验（ELISA）检测microRNA-146 a（miR-146 a）介导的核因子-κ B（NF-κB）信号通路相关蛋白的表达，探讨针刺的抗炎作用机制。注射D-半乳糖（D-gal）的大鼠显示明显的骨骼肌萎缩，横截面积和纤维直径显着减少。相反，针刺治疗减轻了骨骼肌萎缩的这些标志，减轻了线粒体畸变和骨骼肌细胞凋亡在D-半乳糖大鼠。此外，针刺还可下调D-gal大鼠骨骼肌炎症因子的过度表达，影响miR-146 a及其靶基因的表达，抑制NF-κB核翻译。针刺可以改善骨骼肌萎缩，其作用可能与控制线粒体功能调节和抑制炎症有关。
Sarcopenia during aging is closely linked to sterile, low-grade, chronic inflammation. However, considering the increasingly aging global population, the effectiveness of existing treatments for sarcopenia is not exact, and acupuncture, as an effective anti-inflammatory therapy, has the potential to treat it. Fifty Sprague-Dawley rats were randomly allocated into five groups, including Control group, D-galactose (D-gal) group, D-gal + acupuncture (DA) group, D-gal + non-acupoint (DN) group and D-gal amino acid mixture (DAA) group. An aging rat was model constructed using D-gal for 12 weeks. Rats in the control group received 0.9% physiological saline daily. Treatment groups were acupunctured or given amino acid mixture interventions daily, and lasted for last 4 consecutive weeks. The effects of acupuncture were evaluated by the hematoxylin and eosin staining (H&E), transmission electron microscopic (TEM) examination and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays. The anti-inflammatory mechanism of acupuncture was studied by using the expressions of microRNA-146a (miR-146a) mediated nuclear factor-kappa B (NF-κB) signaling pathway-related proteins were detected by immunofluorescence, western blotting, quantitative real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). Rats injected by D-galactose (D-gal) revealed apparent skeletal muscle atrophy with significantly reduced cross-sectional area and fiber diameter. In contrast, acupuncture treatment alleviated these hallmarks of skeletal muscle atrophy and mitigated the mitochondrial aberrations and skeletal muscle apoptosis in D-gal rats. In addition, acupuncture also downgraded the overexpression of inflammatory factors in skeletal muscle, influenced miR-146a and the target genes level, and inhibited NF-κB nuclear translation in D-gal rats. Acupuncture may ameliorate skeletal muscle atrophy, and its effects may be associated with the control of mitochondrial function regulation and the suppression of inflammation.
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影响因子：
5.5
作者：
Carter, Heather N.;Kim, Yuho;Hood, David A.
通讯作者：
Hood, David A.
影响因子：
29
作者：
Baker RG;Hayden MS;Ghosh S
通讯作者：
Ghosh S
DOI：
10.1126/science.1201940
发表时间：
2011-08-26
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Green DR;Galluzzi L;Kroemer G
通讯作者：
Kroemer G
Ampelopsin 通过激活 AMPK/SIRT1/PGC-1α 信号级联来减轻 d-gal 诱导的衰老大鼠骨骼肌萎缩。
影响因子：
7.5
作者：
Kou, Xianjuan;Li, Jie;Chen, Ning
通讯作者：
Chen, Ning
影响因子：
4.1
作者：
Helenius, M;Hanninen, M;Salminen, A
通讯作者：
Salminen, A