Cytokine response after severe respiratory syncytial virus bronchiolitis in early life.
Cytokine response after severe respiratory syncytial virus bronchiolitis in early life.
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DOI：
10.1016/j.jaci.2008.07.010
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发表时间：
2008-10
影响因子：
14.2
通讯作者：
Bacharier, Leonard B.
中科院分区：
文献类型：
--
作者：
Castro, Mario;Schweiger, Toni;Yin-DeClue, Huiquing;Ramkumar, Thiruvamoor P.;Christie, Chandrika;Zheng, Jie;Cohen, Rebecca;Schechtman, Kenneth B.;Strunk, Robert;Bacharier, Leonard B.
病毒感染后的免疫反应通常涉及 Th1 介导的反应；然而，严重的呼吸道合胞病毒 (RSV) 感染似乎与哮喘（一种以 Th2 为主的表型）的发生有关。了解儿童随着时间的推移对严重 RSV 感染的早期和后续免疫反应。 206 名之前健康且因严重 RSV 细支气管炎住院的婴儿被纳入一项名为早期 RSV 细支气管炎 (RBEL) 研究的前瞻性队列。 RSV 感染后以及 2、4 和 6 岁时立即获得外周血 T 细胞，用 PMA 和离子霉素刺激，并通过流式细胞术和实时 PCR 分析白细胞介素 (IL)-2、-4 和 - 13 以及干扰素-γ (IFN-γ)。 48% (n=97) 的儿童在 6 岁时出现哮喘（经医生诊断），48% (n=97) 患有湿疹。32%（n=150 人中的 48 名）在 3 岁时出现过敏。患有哮喘的儿童在 6 岁时的 IL-13 表达低于未患有哮喘的儿童 (p=0.001)。随访期间，IFN-γ、IL-2 和 -4 水平不因哮喘或湿疹状态而异（均 p>0.05）。过敏致敏与随访期间细胞因子水平的差异无关（所有p>0.05）。生命早期严重的 RSV 感染与哮喘和湿疹的高发病率有关。与预期相反，患有哮喘、湿疹或过敏性过敏的患者随后的免疫发展与外周血中的 Th2 表型无关。
Immune response following viral infection usually involves Th1-mediated response; however, severe respiratory syncytial virus (RSV) infection appears to be associated with the development of asthma, a Th2-predominant phenotype. To understand the early and subsequent immunologic response to a serious RSV infection in children over time. 206 previously healthy infants hospitalized with severe RSV bronchiolitis were enrolled in a prospective cohort called the RSV Bronchiolitis in Early Life (RBEL) study. Peripheral blood T cells were obtained immediately following RSV infection and at 2, 4 and 6 years of age, stimulated with PMA and ionomycin, and analyzed for interleukin (IL)-2, -4, and - 13 and interferon-γ (IFN-γ) by flow cytometry and real time PCR. 48% (n=97) of the children developed asthma (physician-diagnosed) and 48% (n=97) had eczema by age 6. 32% (n=48 of 150) developed allergic sensitization by 3 yrs of age. Children with asthma had lower IL-13 expression at 6 yrs of age than those without (p=0.001). IFN-γ, IL-2 and -4 levels did not differ by asthma or eczema status during follow-up (all p>0.05). Allergic sensitization was not associated with differences in cytokine levels during follow-up (all p>0.05). Severe RSV infection early in life is associated with a high incidence of asthma and eczema. Contrary to expectations, subsequent immunologic development in those who developed asthma, eczema or allergic sensitization was not associated with a Th2 phenotype in the peripheral blood.
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DOI：
10.1097/01.asn.0000071514.36428.61
发表时间：
2003-07-01
影响因子：
13.6
作者：
Cooper, AC;Mikhail, A;MacDougall, IC
通讯作者：
MacDougall, IC
DOI：
10.1164/rccm.200210-1148oc
发表时间：
2003-09-15
影响因子：
24.7
作者：
Legg, JP;Hussain, IR;Warner, JO
通讯作者：
Warner, JO
影响因子：
3.8
作者：
Overbergh, L;Valckx, D;Mathieu, C
通讯作者：
Mathieu, C
影响因子：
4.3
作者：
Chipeta, J;Komada, Y;Sakurai, M
通讯作者：
Sakurai, M
DOI：
10.1084/jem.20040035
发表时间：
2004-07-05
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
de Heer HJ;Hammad H;Soullié T;Hijdra D;Vos N;Willart MA;Hoogsteden HC;Lambrecht BN
通讯作者：
Lambrecht BN