Proteomic profiling of human plasma exosomes identifies PPARgamma as an exosome-associated protein.
Proteomic profiling of human plasma exosomes identifies PPARgamma as an exosome-associated protein.
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DOI：
10.1016/j.bbrc.2008.11.050
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发表时间：
2009-01-16
影响因子：
3.1
通讯作者：
Levine, Stewart J.
中科院分区：
文献类型：
--
作者：
Looze, Christopher;Yui, David;Leung, Lester;Ingham, Matthew;Kaler, Maryann;Yao, Xianglan;Wu, Wells W.;Shen, Rong-Fong;Daniels, Mathew P.;Levine, Stewart J.
外来体是作为无细胞细胞间通讯机制从细胞释放的纳米囊泡。从血浆外泌体蛋白质组中仅鉴定了有限数量的蛋白质。在这里，我们开发了一个多步骤的分馏方案，结合凝胶排阻色谱法，通过连续蔗糖梯度的速率区带离心，和高速离心，从人血浆中纯化外泌体。通过SDS-PAGE分离外泌体相关蛋白，并通过LC-MS/MS鉴定了66个蛋白，其中包括细胞和细胞外蛋白。此外，我们鉴定并表征了过氧化物酶体增殖物激活受体-γ（PPARγ），一种调节脂肪细胞分化和增殖以及免疫和炎症细胞功能的核受体，作为血浆来源的外泌体的新组分。鉴于外泌体作为细胞间信使的重要作用，发现PPARγ作为人血浆外泌体的组分确定了核受体旁分泌转移的潜在新途径。
Exosomes are nanovesicles that are released from cells as a mechanism of cell-free intercellular communication. Only a limited number of proteins have been identified from the plasma exosome proteome. Here, we developed a multi-step fractionation scheme incorporating gel exclusion chromatography, rate zonal centrifugation through continuous sucrose gradients, and high-speed centrifugation to purify exosomes from human plasma. Exosome-associated proteins were separated by SDS-PAGE and 66 proteins were identified by LC-MS/MS, which included both cellular and extracellular proteins. Furthermore, we identified and characterized peroxisome proliferator-activated receptor-γ (PPARγ), a nuclear receptor that regulates adipocyte differentiation and proliferation, as well as immune and inflammatory cell functions, as a novel component of plasma-derived exosomes. Given the important role of exosomes as intercellular messengers, the discovery of PPARγ as a component of human plasma exosomes identifies a potential new pathway for the paracrine transfer of nuclear receptors.
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影响因子：
4.4
作者：
Wang, GH;Wu, WW;Shen, RF
通讯作者：
Shen, RF
DOI：
10.1016/j.bbrc.2007.12.011
发表时间：
2008-02-08
影响因子：
3.1
作者：
Zhang, Jing;Hawari, Feras I.;Levine, Stewart J.
通讯作者：
Levine, Stewart J.
影响因子：
--
作者：
Thery, Clotilde;Amigorena, Sebastian;Clayton, Aled
通讯作者：
Clayton, Aled
影响因子：
4.4
作者：
Caby, MP;Lankar, D;Bonnerot, C
通讯作者：
Bonnerot, C
影响因子：
21.3
作者：
Raiborg, C;Bache, KG;Stenmark, H
通讯作者：
Stenmark, H