Selection of optimal epoch duration in assessment of rodent sleep-wake profiles

Selection of optimal epoch duration in assessment of rodent sleep-wake profiles
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评估啮齿类动物睡眠觉醒曲线时最佳历时持续时间的选择

DOI:
10.1111/j.1479-8425.2010.00484.x
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发表时间:
2011
影响因子:
1.1
通讯作者:
Qu WM
Qu WM
中科院分区:
医学4区
文献类型:
--
作者:
Yan MM;Xu XH;Huang ZL;Yao MH;Urade Y;Qu WM

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睡眠研究中遇到的主要不便之一是脑电图(EEG)分析所涉及的耗时劳动。历元持续时间的选择至关重要,并且与脑电图分析的结果高度相关。这项研究的目的是找到最佳的脑电图历元持续时间,从而准确地揭示动物的睡眠-觉醒曲线,并减轻研究人员对啮齿动物睡眠-觉醒参数的繁重分析。我们分析了具有常用历元持续时间(4、8、10、20 和 30 秒)的小鼠和大鼠脑电图信号,并在睡眠和觉醒量、发作次数和持续时间、睡眠-觉醒阶段转换次数和脑电图功率谱方面比较了所得的睡眠-觉醒曲线。在我们使用的 5 个时期持续时间中,觉醒、快速眼动 (REM) 和非快速眼动睡眠的数量和 EEG 功率密度没有统计学差异。然而,纪元越短,阶段转换越多,剧集数量也越大。实验的目的应该是确定实验最佳历元长度的主要标准。当需要每个阶段的数量和EEG信号的功率密度时,30秒的历元持续时间是合适的,以节省时间;所获得的结果与对其他历元持续时间的分析发现的结果没有什么不同。如果要考虑阶段转换、持续时间和发作次数,建议将 4 秒历元持续时间作为分析的最佳选择。
One of the major inconveniences encountered in sleep studies is the time-consuming labor involved in electroencephalography (EEG) analysis. The choice of epoch duration is of prime importance and is highly related to the outcome of EEG analysis. This study was designed to find the optimal EEG epoch duration that accurately reveals the sleep-wake profiles of animals and relieve researchers of the laborious analysis of rodent sleep-wake parameters. We analyzed mouse and rat EEG signals with commonly used epoch durations (4, 8, 10, 20, and 30 s) and compared the resulting sleep-wake profiles in terms of amounts of sleep and wakefulness, number and duration of episodes, number of sleep-wake stage transitions, and EEG power spectra. There were no statistical differences in the amount and EEG power density of wakefulness, rapid eye movement (REM) and non-REM sleep among the 5 epoch durations we used. However, the shorter the epoch, the more numerous the stage transitions and the larger the episode number. The purpose of an experiment should be a main criterion for determining the optimal epoch length for the experiment. When the amount of each stage and power density of EEG signals are desired, a 30-s epoch duration is appropriate, to save time; and the results obtained are no different from those found by analysis of other epoch durations. If stage transition and duration and number of episodes are to be considered, a 4-s epoch duration is suggested as the best choice for analysis.
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