Soluble antigen derived from IV larva of Angiostrongylus cantonensis promotes chitinase-like protein 3 (Chil3) expression induced by interleukin-13
Soluble antigen derived from IV larva of Angiostrongylus cantonensis promotes chitinase-like protein 3 (Chil3) expression induced by interleukin-13
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广州管圆线虫 IV 幼虫来源的可溶性抗原促进白细胞介素 13 诱导的几丁质酶样蛋白 3 (Chil3) 表达
DOI：
10.1007/s00436-016-5135-x
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发表时间：
2016-06
期刊：
影响因子：
--
通讯作者：
Zhongdao Wu
中科院分区：
文献类型：
--
作者：
Zilong Yu;Zhiyue Lv;Xi Sun;Zhongdao Wu
广州管圆线虫病由广州管圆线虫（A.广东嗜酸性粒细胞性脑膜炎是一种新出现的食源性寄生虫病，基本上指嗜酸性粒细胞性脑膜炎。几丁质酶样蛋白3（Chil 3）是几丁质酶样蛋白家族的一员，对嗜酸性粒细胞具有趋化活性。广东话。Chil 3高表达的机制及与A. cantonensis和Chil 3的报道较少。为了了解Chil 3在A.用广州曲霉可溶性抗原刺激RAW 264.7和BV 2细胞株，检测其Chil 3的表达水平。通过qPCR和ELISA方法检测广州小蠊。探讨Chil 3在A.方法：采用体外培养的方法，分离培养大鼠脑单个核细胞（BMNCs），采用transwell法和流式细胞仪检测Chil 3对嗜酸性粒细胞的趋化活性。此外，我们通过注射rmChil 3来治疗感染小鼠，然后对感染小鼠和治疗小鼠脑中的幼虫数量进行计数，以检查蠕虫和Chil 3之间的关联。结果表明，A.结果表明，白细胞介素13（IL-13）诱导巨噬细胞和小胶质细胞系表达Chil 3，而白细胞介素13（IL-13）诱导巨噬细胞和小胶质细胞系表达Chil 3，但白细胞介素13（IL-13）诱导巨噬细胞和小胶质细胞系表达Chil 3。因此，我们推测可溶性抗原可促进Chil 3的高表达。结论：广州曲霉毒素可能是引起小鼠脑内嗜酸性粒细胞严重浸润的原因。广州线虫感染
Angiostrongyliasis caused by Angiostrongylus cantonensis (A. cantonensis) is an emerging food-borne parasitic disease, which refers basically to eosinophilic meningitis. Chitinase-like protein 3 (Chil3), a member of chitinase-like protein family which has chemotactic activity for eosinophils, is reported to be highly upregulated in brain of mouse infected with A. cantonensis. The mechanisms of high expression of Chil3 and the association between A. cantonensis and Chil3 are rarely reported. In order to understand the mechanism of high expression of Chil3 in A. cantonensis-infected mouse, we measured the level of Chil3 in RAW 264.7 and BV2 cell lines stimulated with soluble antigen of A. cantonensis by qPCR and ELISA. To explore the role of Chil3 in inflammation caused by A. cantonensis, we extracted and cultured brain mononuclear cells (BMNCs) and detected the eosinophil chemotactic activity of Chil3 using transwell assay and flow cytometer. Furthermore, we treated the infected mice by injection with rmChil3 and then counted the number of larvae in brains of infected mice and treated mice to examine the association between the worm and Chil3. Our results showed the soluble antigen from A. cantonensis could promote the Chil3 expression in macrophage and microglial cell lines induced by interleukin-13. In conclusion, we supposed that high expression of Chil3 enhanced by soluble antigens from A. cantonensis might be the reason of serious eosinophil infiltration in mouse brain after A. cantonensis infection.
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DOI：
--
发表时间：
1987
期刊：
International archives of allergy and applied immunology
影响因子：
--
作者：
M. Owhashi;Y. Nawa
通讯作者：
M. Owhashi;Y. Nawa
DOI：
10.1051/parasite/1935134312
发表时间：
1935
期刊：
Annales de parasitologie humaine et comparée
影响因子：
--
作者：
H.-T. Chen
通讯作者：
H.-T. Chen
影响因子：
25
作者：
Miron, Veronique E.;Boyd, Amanda;Zhao, Jing-Wei;Yuen, Tracy J.;Ruckh, Julia M.;Shadrach, Jennifer L.;van Wijngaarden, Peter;Wagers, Amy J.;Williams, Anna;Franklin, Robin J. M.;Ffrench-Constant, Charles
通讯作者：
Ffrench-Constant, Charles
影响因子：
--
作者：
L. Rosen;J. Laigret;S. Bories
通讯作者：
L. Rosen;J. Laigret;S. Bories
DOI：
10.1074/jbc.275.2.1279
发表时间：
2000-01
期刊：
The Journal of Biological Chemistry
影响因子：
--
作者：
M. Owhashi;H. Arita;N. Hayai
通讯作者：
M. Owhashi;H. Arita;N. Hayai