Comparative proteomic analysis of embryos between a maize hybrid and its parental lines during early stages of seed germination.
Comparative proteomic analysis of embryos between a maize hybrid and its parental lines during early stages of seed germination.
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DOI：
10.1371/journal.pone.0065867
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Sun Q
中科院分区：
文献类型：
--
作者：
Guo B;Chen Y;Zhang G;Xing J;Hu Z;Feng W;Yao Y;Peng H;Du J;Zhang Y;Ni Z;Sun Q
尽管杂种优势在农业上有商业用途，但人们对杂种优势的分子基础知之甚少。结果表明，玉米杂交种综3/87-1在胚根萌发方面表现出较早的发病或杂种优势。为了深入了解杂种优势在胚根萌发中的潜在机制，对杂交种及其亲本进行了差异蛋白质组分析。结果表明，在干种胚和吸胀24 h的种子胚胎中，杂交种与其亲本间差异表达的蛋白质点数分别为134个和191个，其中47.01%(63/134)和34.55%(66/191)的蛋白质点呈现非加性表达模式。值得注意的是，吸胀24 h的种子胚胎中有54.55%的非加性积累的蛋白质显示在或等于较高亲本模式的水平。此外，还鉴定了155个差异表达蛋白质点，分为转录与翻译、能量与代谢、信号转导、疾病与防御、贮藏蛋白、转座元件、细胞生长与分裂和未分类蛋白质等8个功能类。此外，在F1中上调的蛋白质之一是ZmACT2，它是拟南芥ACT7(AtACT7)的同源物。由AtACT7启动子驱动的ZmACT2表达部分补充了Atact7突变体的低萌发表型。这些结果表明，两个亲本之间的杂交可以引起多种蛋白质表达的变化，并得出结论，杂交种在翻译水平上的基因表达模式的改变可能是造成所观察到的杂种优势的原因。
In spite of commercial use of heterosis in agriculture, the molecular basis of heterosis is poorly understood. It was observed that maize hybrid Zong3/87-1 exhibited an earlier onset or heterosis in radicle emergence. To get insights into the underlying mechanism of heterosis in radicle emergence, differential proteomic analysis between hybrid and its parental lines was performed. In total, the number of differentially expressed protein spots between hybrid and its parental lines in dry and 24 h imbibed seed embryos were 134 and 191, respectively, among which 47.01% (63/134) and 34.55% (66/191) protein spots displayed nonadditively expressed pattern. Remarkably, 54.55% of nonadditively accumulated proteins in 24 h imbibed seed embryos displayed above or equal to the level of the higher parent patterns. Moreover, 155 differentially expressed protein spots were identified, which were grouped into eight functional classes, including transcription & translation, energy & metabolism, signal transduction, disease & defense, storage protein, transposable element, cell growth & division and unclassified proteins. In addition, one of the upregulated proteins in F1 hybrids was ZmACT2, a homolog of Arabidopsis thaliana ACT7 (AtACT7). Expressing ZmACT2 driven by the AtACT7 promoter partially complemented the low germination phenotype in the Atact7 mutant. These results indicated that hybridization between two parental lines can cause changes in the expression of a variety of proteins, and it is concluded that the altered pattern of gene expression at translational level in the hybrid may be responsible for the observed heterosis.
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DOI：
10.1073/pnas.132259199
发表时间：
2002-07-09
影响因子：
11.1
作者：
Fu, HH;Dooner, HK
通讯作者：
Dooner, HK
影响因子：
5.4
作者：
Guo, Mei;Rupe, Mary A.;Bowen, Ben
通讯作者：
Bowen, Ben
影响因子：
5.4
作者：
HEIDRICHSOBRINHO, E;CORDEIRO, AR
通讯作者：
CORDEIRO, AR
影响因子：
7.2
作者：
Liu, Po-Pu;Montgomery, Taiowa A.;Carrington, James C.
通讯作者：
Carrington, James C.
影响因子：
7.2
作者：
Gilliland, LU;Pawloski, LC;Meagher, RB
通讯作者：
Meagher, RB