Bacterial endosymbiosis is widely present among zygomycetes but does not contribute to the pathogenesis of mucormycosis.
Bacterial endosymbiosis is widely present among zygomycetes but does not contribute to the pathogenesis of mucormycosis.
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DOI：
10.1086/591461
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发表时间：
2008-10-01
期刊：
影响因子：
--
通讯作者：
Spellberg B
中科院分区：
文献类型：
--
作者：
Ibrahim AS;Gebremariam T;Liu M;Chamilos G;Kontoyiannis D;Mink R;Kwon-Chung KJ;Fu Y;Skory CD;Edwards JE Jr;Spellberg B
环境中的根霉分离株已被证明含有一种细菌内共生菌(Burkholderia)，它能产生一种植物真菌毒素rhixozin。我们试图确定内共生菌产生根霉毒素在毛霉病发病机制中的作用。聚合酶链式反应在28株接合菌临床分离株中有15株(54%)可鉴定出内共生菌，其中33%的菌株与伯克霍尔德氏菌16S rDNA的≥同源性为87%。高效液相色谱分析证实含有细菌的真菌菌丝提取液中含有根霉毒素。然而，有或没有内共生体的真菌菌株在体外引起内皮细胞损伤的能力没有区别，抗生素介导的内共生菌的根除和根毒素的产生也不会降低真菌菌株在小鼠或苍蝇中的毒力。综上所述，尽管在包括米根霉在内的接合菌临床分离株中广泛检测到细菌内共生，但在所研究的模型中，我们没有发现细菌内共生菌和根霉毒素与毛霉病发病有关的证据。
Environmental isolates of the fungus Rhizopus have been shown to harbor a bacterial endosymbiont (Burkholderia) that produces rhixozin, a plant mycotoxin. We sought to define the role of rhizoxin production by endosymbionts in the pathogenesis of mucormycosis. Endosymbiotic bacteria were identified by polymerase chain reaction in 15 (54%) of 28 clinical isolates of Zygomycetes, with 33% of the bacterial strains showing ≥87% identity to Burkholderia 16S rDNA. The presence of rhizoxin in myclial extracts from fungi harboring bacteria was confirmed by high-performance liquid chromatography analysis. However, fungal strains with or without endosymbionts did not differ in their ability to cause endothelial cell injury in vitro, nor did antibiotic-mediated eradication of endosymbionts and rhizoxin production decrease the virulence of fungal strains in mice or flies. In summary, although bacterial endosymbiosis is widely detected in clinical isolates of Zygomycetes, including Rhizopus oryzae strains, we found no evidence that bacterial endosymbionts and rhizoxin contribute to the pathogenesis of mucormycosis in the models studied.
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影响因子：
4.9
作者：
Ibrahim, AS;Avanessian, V;Edwards, JE
通讯作者：
Edwards, JE
影响因子：
11.8
作者：
Reed, Caitlin;Bryant, Richard;Spellberg, Brad
通讯作者：
Spellberg, Brad
影响因子：
8.8
作者：
McLeod, HL;Murray, LS;Verweij, J
通讯作者：
Verweij, J
影响因子：
15
作者：
Scherlach, Kirstin;Partida-Martinez, Laila P.;Hertweck, Christian
通讯作者：
Hertweck, Christian
影响因子：
15.9
作者：
JAFFE, EA;NACHMAN, RL;MINICK, CR
通讯作者：
MINICK, CR