Diverse viral glycoproteins as well as CD4 co-package into the same human immunodeficiency virus (HIV-1) particles.
Diverse viral glycoproteins as well as CD4 co-package into the same human immunodeficiency virus (HIV-1) particles.
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多种病毒糖蛋白以及CD4共包装到相同的人类免疫缺陷病毒（HIV-1）颗粒中。
DOI：
10.1186/1742-4690-11-28
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发表时间：
2014-04-03
期刊：
影响因子：
3.3
通讯作者：
Johnson MC
中科院分区：
文献类型：
--
作者：
Gregory DA;Olinger GY;Lucas TM;Johnson MC
逆转录病毒从细胞膜上出芽时不仅可以获得自身的糖蛋白，还可以获得一些细胞内和外源病毒的糖蛋白。这些非天然糖蛋白中的许多被主动募集到出芽病毒体，特别是其他病毒糖蛋白。这一观察结果表明，可能有一个保守的机制，招募糖蛋白进入病毒。如果使用保守的机制，不同的糖蛋白应该定位到一个单一的出芽逆转录病毒颗粒。另一方面，如果病毒糖蛋白具有不同的募集机制，则不同的糖蛋白可以分离成不同的颗粒。为了确定不同糖蛋白之间是否发生共包装，我们设计了一种结合病毒体抗体捕获和基于荧光素酶报告基因的感染性测定的测定。将病毒粒子与针对一种糖蛋白的抗体结合到平板上，然后用仅允许第二种糖蛋白进入的细胞测量感染性。我们测试了来自HIV、鼠白血病病毒（MLV）、劳斯肉瘤病毒（RSV）、水泡性口炎病毒（VSV）和埃博拉病毒的糖蛋白配对。结果表明，被积极招募到病毒粒子中的糖蛋白彼此有效地共同包装。我们还测试了细胞蛋白质，发现CD 4在主动募集和有效的共包装之间也有类似的相关性，但其他细胞蛋白质没有。被积极纳入HIV-1病毒粒子的糖蛋白被有效地共同包装到相同的病毒颗粒中，这表明相同的一般招募机制可能在许多病毒中起作用。
Retroviruses can acquire not only their own glycoproteins as they bud from the cellular membrane, but also some cellular and foreign viral glycoproteins. Many of these non-native glycoproteins are actively recruited to budding virions, particularly other viral glycoproteins. This observation suggests that there may be a conserved mechanism underlying the recruitment of glycoproteins into viruses. If a conserved mechanism is used, diverse glycoproteins should localize to a single budding retroviral particle. On the other hand, if viral glycoproteins have divergent mechanisms for recruitment, the different glycoproteins could segregate into different particles. To determine if co-packaging occurs among different glycoproteins, we designed an assay that combines virion antibody capture and a determination of infectivity based on a luciferase reporter. Virions were bound to a plate with an antibody against one glycoprotein, and then the infectivity was measured with cells that allow entry only with a second glycoprotein. We tested pairings of glycoproteins from HIV, murine leukemia virus (MLV), Rous sarcoma virus (RSV), vesicular stomatitis virus (VSV), and Ebola virus. The results showed that glycoproteins that were actively recruited into virions were co-packaged efficiently with each other. We also tested cellular proteins and found CD4 also had a similar correlation between active recruitment and efficient co-packaging, but other cellular proteins did not. Glycoproteins that are actively incorporated into HIV-1 virions are efficiently co-packaged into the same virus particles, suggesting that the same general mechanism for recruitment may act in many viruses.
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影响因子：
6.7
作者：
Ivanchenko S;Godinez WJ;Lampe M;Kräusslich HG;Eils R;Rohr K;Bräuchle C;Müller B;Lamb DC
通讯作者：
Lamb DC
影响因子：
5.6
作者：
Checkley MA;Luttge BG;Freed EO
通讯作者：
Freed EO
影响因子：
3.7
作者：
Kothe, Denise L.;Decker, Julie M.;Hahn, Beatrice H.
通讯作者：
Hahn, Beatrice H.
影响因子：
16
作者：
Boyko, Vitaly;Leavitt, Maria;Hu, Wei-Shau
通讯作者：
Hu, Wei-Shau
影响因子：
5.4
作者：
Mammano, F;Salvatori, F;Gottlinger, HG
通讯作者：
Gottlinger, HG