Xylan extraction from pretreated sugarcane bagasse using alkaline and enzymatic approaches.
Xylan extraction from pretreated sugarcane bagasse using alkaline and enzymatic approaches.
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DOI：
10.1186/s13068-017-0981-z
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发表时间：
2017
影响因子：
6.3
通讯作者：
Milagres AMF
中科院分区：
文献类型：
--
作者：
Sporck D;Reinoso FAM;Rencoret J;Gutiérrez A;Del Rio JC;Ferraz A;Milagres AMF
新的生物炼制概念是必要的，以推动工业使用木质纤维素生物质组分。在葡聚糖组分的酶促水解之前回收木聚糖是一种为半纤维素级分增值的方式，其可用于造纸、制药和食品工业。半纤维素去除还可促进随后的纤维素分解葡聚糖水解。采用碱-亚硫酸盐化学热机械法对甘蔗渣进行预处理，以利于后续的酶法或碱法提取木聚糖。碱提取方法得到53%（w/w）木聚糖回收率。酶促方法提供了22%（w/w）的有限产率，但产生了具有最低的木质素和葡聚糖组分污染的木聚糖。所有提取的木聚糖阿拉伯糖侧基和乙酰化的情况下。2D-NMR数据表明，O-甲基-葡萄糖醛酸和p-香豆酸酯的存在下，只有酶法提取木聚糖。使用酶促方法分离的木聚糖产生分子量（Mw）低于6 kDa的产物。在碱分离的木聚糖中检测到较高的Mw值。木聚糖的碱提取提供了富含葡聚糖的固体，其易于用低纤维素酶负荷水解，产生具有高葡萄糖/木糖比的水解产物。在纤维素部分的酶水解之前去除半纤维素被证明是增加甘蔗渣生物精炼价值的有效方式。根据提取方法可以获得具有不同产率、纯度和结构的木聚糖。酶法提取工艺以低收率生产高纯度木聚糖，而碱法提取工艺提供了较高的木聚糖收率，同时具有更多的木质素和葡聚糖污染。当用碱性方法进行木聚糖提取时，残余的富含葡聚糖的固体似乎适合于采用低纤维素酶负载的葡萄糖生产。本文的在线版本（10.1186/s13068-017-0981-z）包含补充材料，可供授权用户使用。
New biorefinery concepts are necessary to drive industrial use of lignocellulose biomass components. Xylan recovery before enzymatic hydrolysis of the glucan component is a way to add value to the hemicellulose fraction, which can be used in papermaking, pharmaceutical, and food industries. Hemicellulose removal can also facilitate subsequent cellulolytic glucan hydrolysis. Sugarcane bagasse was pretreated with an alkaline-sulfite chemithermomechanical process to facilitate subsequent extraction of xylan by enzymatic or alkaline procedures. Alkaline extraction methods yielded 53% (w/w) xylan recovery. The enzymatic approach provided a limited yield of 22% (w/w) but produced the xylan with the lowest contamination with lignin and glucan components. All extracted xylans presented arabinosyl side groups and absence of acetylation. 2D-NMR data suggested the presence of O-methyl-glucuronic acid and p-coumarates only in enzymatically extracted xylan. Xylans isolated using the enzymatic approach resulted in products with molecular weights (Mw) lower than 6 kDa. Higher Mw values were detected in the alkali-isolated xylans. Alkaline extraction of xylan provided a glucan-enriched solid readily hydrolysable with low cellulase loads, generating hydrolysates with a high glucose/xylose ratio. Hemicellulose removal before enzymatic hydrolysis of the cellulosic fraction proved to be an efficient manner to add value to sugarcane bagasse biorefining. Xylans with varied yield, purity, and structure can be obtained according to the extraction method. Enzymatic extraction procedures produce high-purity xylans at low yield, whereas alkaline extraction methods provided higher xylan yields with more lignin and glucan contamination. When xylan extraction is performed with alkaline methods, the residual glucan-enriched solid seems suitable for glucose production employing low cellulase loadings. The online version of this article (10.1186/s13068-017-0981-z) contains supplementary material, which is available to authorized users.
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影响因子：
11.2
作者：
Höije, A;Gröndahl, M;Gatenholm, P
通讯作者：
Gatenholm, P
影响因子：
6
作者：
del Rio, Jose C.;Lino, Alessandro G.;Rencoret, Jorge
通讯作者：
Rencoret, Jorge
影响因子：
3.4
作者：
Chimphango, Annie F. A.;van Zyl, Willem H.;Goergens, Johann F.
通讯作者：
Goergens, Johann F.
影响因子：
11.2
作者：
Eguees, Itziar;Stepan, Agnes M.;Labidi, Jalel
通讯作者：
Labidi, Jalel
影响因子：
11.2
作者：
Buranov, Anvar U.;Mazza, G.
通讯作者：
Mazza, G.