High-throughput phenotyping (HTP) identifies seedling root traits linked to variation in seed yield and nutrient capture in field-grown oilseed rape (Brassica napus L.).
High-throughput phenotyping (HTP) identifies seedling root traits linked to variation in seed yield and nutrient capture in field-grown oilseed rape (Brassica napus L.).
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DOI：
10.1093/aob/mcw046
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发表时间：
2016-10-01
期刊：
影响因子：
4.2
通讯作者：
Broadley MR
中科院分区：
文献类型：
--
作者：
Thomas CL;Graham NS;Hayden R;Meacham MC;Neugebauer K;Nightingale M;Dupuy LX;Hammond JP;White PJ;Broadley MR
背景和目的根系性状可以被选择用于作物改良。土壤挖掘等技术可用于在田间筛选根系性状，但仅限于适应田间条件的基因型。本研究的目的是比较低成本，高通量根表型（HTP）技术在受控环境与田间表现，油菜（OSR;甘蓝型油菜）品种。方法以32个14 d龄的优质OSR品种幼苗为材料，采用“袋芯法”HTP系统，测定其主根长（PRL）、侧根长（LRD）和侧根密度（LRD），重复140次。在英国的两个地点，每年使用相同的品种进行6次田间试验，为期3年。在所有六个实验中，在6至8叶阶段挖掘植物用于根和芽的一般活力评估，并确定最终种子产量。从田间实验之一中取样叶的矿物质组成。关键结果HTP系统中的幼苗PRL在6个田间试验中的4个试验中与种子产量相关（r = 0·50、0·50、0·33、0·49; P < 0·05），在5个田间试验中的3个试验中与出苗相关（r = 0·59、0·22、0·49; P < 0·05）。幼苗LRD与叶片中钙（r = 0.46; P <0.01）和锌（r = 0.58; P <0.001）含量呈正相关，但与出苗率、一般早期活力和田间产量无关。结论PRL和田间表现之间的关联一般与早期活力有关。这些根性状可能因此是有限的额外的选择价值，考虑到活力可以很容易地测量芽/冠层。相比之下，LRD不能很容易地在现场进行评估，如果LRD可以提高养分吸收，那么它可能是可能的使用HTP系统来筛选这一特性在精英和更遗传多样性，非现场适应OSR。
Background and Aims Root traits can be selected for crop improvement. Techniques such as soil excavations can be used to screen root traits in the field, but are limited to genotypes that are well-adapted to field conditions. The aim of this study was to compare a low-cost, high-throughput root phenotyping (HTP) technique in a controlled environment with field performance, using oilseed rape (OSR; Brassica napus) varieties. Methods Primary root length (PRL), lateral root length and lateral root density (LRD) were measured on 14-d-old seedlings of elite OSR varieties (n = 32) using a ‘pouch and wick’ HTP system (∼40 replicates). Six field experiments were conducted using the same varieties at two UK sites each year for 3 years. Plants were excavated at the 6- to 8-leaf stage for general vigour assessments of roots and shoots in all six experiments, and final seed yield was determined. Leaves were sampled for mineral composition from one of the field experiments. Key Results Seedling PRL in the HTP system correlated with seed yield in four out of six (r = 0·50, 0·50, 0·33, 0·49; P < 0·05) and with emergence in three out of five (r = 0·59, 0·22, 0·49; P < 0·05) field experiments. Seedling LRD correlated positively with leaf concentrations of some minerals, e.g. calcium (r = 0·46; P < 0·01) and zinc (r = 0·58; P < 0·001), but did not correlate with emergence, general early vigour or yield in the field. Conclusions Associations between PRL and field performance are generally related to early vigour. These root traits might therefore be of limited additional selection value, given that vigour can be measured easily on shoots/canopies. In contrast, LRD cannot be assessed easily in the field and, if LRD can improve nutrient uptake, then it may be possible to use HTP systems to screen this trait in both elite and more genetically diverse, non-field-adapted OSR.
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影响因子：
6.9
作者：
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通讯作者：
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影响因子：
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作者：
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作者：
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影响因子：
5.4
作者：
Chin, Joong Hyoun;Lu, Xiaochun;Heuer, Sigrid
通讯作者：
Heuer, Sigrid