Phylogenetic analysis of glucosyltransferases and implications for the coevolution of mutans streptococci with their mammalian hosts.
Phylogenetic analysis of glucosyltransferases and implications for the coevolution of mutans streptococci with their mammalian hosts.
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葡萄糖基转移酶的系统发育分析及其对肌肉链球菌与哺乳动物宿主的共同进化的影响。
DOI：
10.1371/journal.pone.0056305
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Caufield PW
中科院分区：
文献类型：
--
作者：
Argimón S;Alekseyenko AV;DeSalle R;Caufield PW
葡萄糖基转移酶（Gtfs）催化蔗糖合成葡聚糖，由几种乳酸菌产生。口腔细菌变形链球菌通过三个Gtfs的作用产生大量葡聚糖。GtfD合成水溶性葡聚糖（WSG）， GtfB合成水不溶性葡聚糖（WIG）， GtfC主要合成WIG和WSG。这些酶，尤其是那些合成WIG的酶，因为它们在牙菌斑形成中的作用而引起了人们的特别关注，牙菌斑是变形链球菌生长和产生乳酸的环境，促进了龋齿的形成。我们对从人类口腔分离的几种突变链球菌菌株的gtfB、gtfC和gtfD基因进行了测序，并在黑猩猩和猕猴分离的菌株中寻找它们的同源物。将序列数据与链球菌属、乳酸菌属和白菌属其他细菌的可用Gtf序列进行分析，以深入了解该酶家族的进化史，特别强调S. mutans的Gtf。我们的分析表明，链球菌Gtfs起源于一个共同的祖先祖基因，并根据它们合成的葡聚糖类型扩展形成两个分支。我们还发现，合成WIG的链球菌Gtfs进化支在胎生齿状哺乳动物分化后不久就出现了，这可能有助于牙菌斑的形成和口腔中几种链球菌的建立。两种能够合成WIG的变形链球菌Gtfs， GtfB和GtfC，可能是基因复制事件的产物。我们将这一事件与早期灵长类祖先基因组的分化相吻合。因此，变形链球菌Gtfs的获得和多样化早于现代人类，与饮食中蔗糖摄入量的增加无关。
Glucosyltransferases (Gtfs) catalyze the synthesis of glucans from sucrose and are produced by several species of lactic-acid bacteria. The oral bacterium Streptococcus mutans produces large amounts of glucans through the action of three Gtfs. GtfD produces water-soluble glucan (WSG), GtfB synthesizes water-insoluble glucans (WIG) and GtfC produces mainly WIG but also WSG. These enzymes, especially those synthesizing WIG, are of particular interest because of their role in the formation of dental plaque, an environment where S. mutans can thrive and produce lactic acid, promoting the formation of dental caries. We sequenced the gtfB, gtfC and gtfD genes from several mutans streptococcal strains isolated from the oral cavity of humans and searched for their homologues in strains isolated from chimpanzees and macaque monkeys. The sequence data were analyzed in conjunction with the available Gtf sequences from other bacteria in the genera Streptococcus, Lactobacillus and Leuconostoc to gain insights into the evolutionary history of this family of enzymes, with a particular emphasis on S. mutans Gtfs. Our analyses indicate that streptococcal Gtfs arose from a common ancestral progenitor gene, and that they expanded to form two clades according to the type of glucan they synthesize. We also show that the clade of streptococcal Gtfs synthesizing WIG appeared shortly after the divergence of viviparous, dentate mammals, which potentially contributed to the formation of dental plaque and the establishment of several streptococci in the oral cavity. The two S. mutans Gtfs capable of WIG synthesis, GtfB and GtfC, are likely the product of a gene duplication event. We dated this event to coincide with the divergence of the genomes of ancestral early primates. Thus, the acquisition and diversification of S. mutans Gtfs predates modern humans and is unrelated to the increase in dietary sucrose consumption.
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影响因子：
14.9
作者：
Abascal F;Zardoya R;Telford MJ
通讯作者：
Telford MJ
影响因子：
4.4
作者：
Gregoire, S.;Xiao, J.;Koo, H.
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影响因子：
10.7
作者：
Gu, X
通讯作者：
Gu, X
影响因子：
3.1
作者：
HANADA, N;KURAMITSU, HK
通讯作者：
KURAMITSU, HK
影响因子：
5.8
作者：
Arnold, K;Bordoli, L;Schwede, T
通讯作者：
Schwede, T