Neuroprotective Effect of Macrophage Migration Inhibitory Factor (MIF) in a Mouse Model of Ischemic Stroke.
Neuroprotective Effect of Macrophage Migration Inhibitory Factor (MIF) in a Mouse Model of Ischemic Stroke.
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DOI：
10.3390/ijms23136975
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发表时间：
2022-06-23
影响因子：
5.6
通讯作者：
Kim DY
中科院分区：
文献类型：
--
作者：
Kim JA;Kim YY;Lee SH;Jung C;Kim MH;Kim DY
巨噬细胞迁移抑制因子（MIF）在体内的神经保护作用机制尚不清楚。我们研究了 MIF 是否促进缺血性中风小鼠体内模型的神经功能恢复。采用短暂性大脑中动脉闭塞（MCAO）手术制作缺血性脑卒中小鼠模型。将雄性小鼠分配至假载体组、假MIF组、大脑中动脉闭塞(MCAO)载体组和MCAO+MIF组。在 MCAO 组中进行短暂 MCAO (tMCAO)，并通过脑室内途径施用载体和 MIF。我们评估了神经功能量表、转棒测试和 T2 加权磁共振成像。通过蛋白质印迹法进一步测量微管相关蛋白2（MAP2）、Bcl2和脑源性神经营养因子（BDNF）的表达水平。 MCAO+MIF 组的 Garcia 测试显着高于 MCAO+媒介物组。 MCAO+MIF 组在旋转测试中表现出明显优于 MCAO+载体组的性能，并且与 MCAO 载体组相比，T2 加权 MRI 成像的总梗塞体积显着减少。 MCAO+MIF组中BDNF和MAP2的表达水平往往高于MCAO+媒介物组。 MIF 在体内缺血性中风模型中发挥神经保护作用。 MIF 促进神经功能恢复并保护脑组织免受缺血性损伤，这表明未来有可能成为中风患者的新型治疗药物。
The mechanism of the neuroprotective effect of the macrophage migration inhibitory factor (MIF) in vivo is unclear. We investigated whether the MIF promotes neurological recovery in an in vivo mouse model of ischemic stroke. Transient middle cerebral artery occlusion (MCAO) surgery was performed to make ischemic stroke mouse model. Male mice were allocated to a sham vehicle, a sham MIF, a middle cerebral artery occlusion (MCAO) vehicle, and MCAO+MIF groups. Transient MCAO (tMCAO) was performed in the MCAO groups, and the vehicle and the MIF were administered via the intracerebroventricular route. We evaluated the neurological functional scale, the rotarod test, and T2-weighted magnetic resonance imaging. The expression level of the microtubule-associated protein 2 (MAP2), Bcl2, and the brain-derived neurotrophic factor (BDNF) were further measured by Western blot assay. The Garcia test was significantly higher in the MCAO+MIF group than in the MCAO+vehicle group. The MCAO+MIF group exhibited significantly better performance on the rotarod test than the MCAO+vehicle group, which further had a significantly reduced total infarct volume on T2-weighted MRI imaging than the MCAO vehicle group. Expression levels of BDNF, and MAP2 tended to be higher in the MCAO+MIF group than in the MCAO+vehicle group. The MIF exerts a neuroprotective effect in an in vivo ischemic stroke model. The MIF facilitates neurological recovery and protects brain tissue from ischemic injury, indicating a possibility of future novel therapeutic agents for stroke patients.
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HLA-DRα1-MMOG-35-55治疗实验性自身免疫性脑脊髓炎可减少中枢神经系统炎症，增强M2巨噬细胞频率并促进神经保护作用。
影响因子：
9.3
作者：
Benedek G;Meza-Romero R;Jordan K;Keenlyside L;Offner H;Vandenbark AA
通讯作者：
Vandenbark AA
影响因子：
5.3
作者：
Putcha, GV;Deshmukh, M;Johnson, EM
通讯作者：
Johnson, EM
影响因子：
4.1
作者：
Kawabori M;Yenari MA
通讯作者：
Yenari MA
影响因子：
6.1
作者：
Chang, Min Cheol;Park, Chae Ri;Kim, Dae Yul
通讯作者：
Kim, Dae Yul
DOI：
10.1159/000091705
发表时间：
2006-01-01
期刊：
Cerebrovascular diseases (Basel, Switzerland)
影响因子：
--
作者：
Fisher, Marc
通讯作者：
Fisher, Marc