Validation of MRI biomarkers of hepatic steatosis in the presence of iron overload in the ob/ob mouse.
Validation of MRI biomarkers of hepatic steatosis in the presence of iron overload in the ob/ob mouse.
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DOI：
10.1002/jmri.22890
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发表时间：
2012-04
影响因子：
4.4
通讯作者：
Reeder, Scott B.
中科院分区：
文献类型：
--
作者：
Hines, Catherine D. G.;Agni, Rashmi;Roen, Calista;Rowland, Ian;Hernando, Diego;Bultman, Eric;Horng, Debra;Yu, Huanzhou;Shimakawa, Ann;Brittain, Jean H.;Reeder, Scott B.
在脂肪变性和铁过载的动物模型中验证一种T2*校正方法用于肝脏脂肪定量的实用性和性能。
将患有低（n = 6）、中（n = 6）和高（n = 8）水平脂肪变性的小鼠麻醉，并使用基于化学位移的脂肪 - 水分离方法进行成像，以获得MRI脂肪分数测量值。在两次注射超顺磁性氧化铁前后均进行成像，以造成肝脏铁过载。在有和没有T2*校正的情况下重建脂肪分数图。在每次注射后比较有和没有T2*校正的脂肪分数以及T2*测量值。采集肝脏组织，并将成像结果与甘油三酯提取和组织学分级进行比较。
MRI脂肪分数与基于组织的脂肪定量之间具有良好的相关性。注射超顺磁性氧化铁导致R2*（= 1/T2*）增加。当使用T2*校正时，测量的脂肪分数不受铁存在的影响，而在没有T2*校正的情况下，测量的脂肪分数显著增加。
在脂肪变性和铁过载的动物模型中验证了使用基于化学位移且经T2*校正的脂肪 - 水分离方法测量的肝脏脂肪分数。T2*校正能够在有铁和无铁的情况下都可靠地估计脂肪分数，并且对于准确的肝脏脂肪定量是必要的。
To validate the utility and performance of a T2* correction method for hepatic fat quantification in an animal model of both steatosis and iron overload. Mice with low (n=6), medium (n=6) and high (n=8) levels of steatosis were sedated and imaged using a chemical shift-based fat-water separation method to obtain MRI fat-fraction measurements. Imaging was performed before and after each of two superparamagnetic iron oxide injections to create hepatic iron overload. Fat-fraction maps were reconstructed with and without T2* correction. Fat-fraction with and without T2* correction, and T2* measurements were compared after each injection. Liver tissue was harvested and imaging results were compared to triglyceride extraction and histology grading. Excellent correlation was seen between MRI fat-fraction and tissue-based fat quantification. Injections of SPIOs led to increases in R2* (=1/T2*). Measured fat-fraction was unaffected by the presence of iron when T2* correction was used, whereas measured fat-fraction dramatically increased without T2* correction. Hepatic fat-fraction measured using a T2*-corrected chemical shift-based fat-water separation method was validated in an animal model of steatosis and iron overload. T2* correction enables robust fat-fraction estimation in both the presence and absence of iron, and is necessary for accurate hepatic fat quantification.
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影响因子：
19.7
作者：
Hines, Catherine D. G.;Yu, Huanzhou;Reeder, Scott B.
通讯作者：
Reeder, Scott B.
影响因子：
13.5
作者：
Browning, JD;Szczepaniak, LS;Hobbs, HH
通讯作者：
Hobbs, HH
影响因子：
4.4
作者：
Guiu, Boris;Petit, Jean-Michel;Krause, Denis
通讯作者：
Krause, Denis
DOI：
10.1016/j.mric.2010.08.014
发表时间：
2010-08
影响因子：
1.6
作者：
Sirlin, Claude B.;Reeder, Scott B.
通讯作者：
Reeder, Scott B.
影响因子：
3.3
作者：
Reeder, SB;Pineda, AR;Pelc, NJ
通讯作者：
Pelc, NJ