Juvenile hormone action to suppress gene transcription and influence message stability.

Juvenile hormone action to suppress gene transcription and influence message stability.
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保幼激素抑制基因转录并影响信息稳定性的作用。

DOI:
10.1002/dvg.1020140410
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发表时间:
1993
期刊:
Developmental genetics
影响因子:
--
通讯作者:
Schelling,D
Schelling,D
中科院分区:
--
文献类型:
--
作者:
Jones,G;Venkataraman,V;Manczak,M;Schelling,D

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本研究通过比较分析激素对其表达的控制作用和水平,研究了通常只在虫蛹中高丰度表达的蛋白。核运行分析表明,血青素超家族中的一些相关蛋白(即酸性蛋白[AJHSP1]和两个碱性蛋白[BJHSP1, BJHSP2])的转录被幼体激素(JH)特异性抑制,而该家族中另一个与变态相关的成员(芳烃蛋白)的转录对JH不特异性敏感。在高JH条件下,那些被JH转录下调的成员mRNA的稳定性下降。虽然每一种蛋白都被蛹前脂肪体吸收,但只有两种基本蛋白从蛹前血淋巴中被定量清除。研究的JH敏感蛋白似乎编码在单拷贝基因中,而不是立即并置在基因组中。这些和先前的研究现在允许更全面地了解涉及转录,mRNA稳定性,翻译和蛋白质清除的不同机制组合,这些机制调节这些变态相关蛋白。©1993Wiley‐Liss, Inc。
Proteins normally expressed in high abundance only at larval‐pupal metamorphosis inTrichoplusia niwere examined in a comparative analysis of the role and level of hormonal control of their expression. Some related proteins in the hemocyanin‐superfamily (i.e., an acidic protein [AJHSP1] and two basic proteins [BJHSP1, BJHSP2]) were shown by nuclear run‐on analysis to be specifically transcriptionally suppressed by juvenile hormone (JH), while transcription of another member of that family which is also metamorphosis‐associated (arylphorin) was not specifically sensitive to JH. The stability of the mRNA for those members transcriptionally down‐regulated by JH appeared to decrease under high JH conditions. While each protein was resorbed to some extent by the prepupal fat body, only the two basic proteins were quantitatively cleared from prepupal hemolymph. The JH‐sensitive proteins studied appear to be encoded in single copy genes not immediately juxtaposed in the genome. These and previous studies now permit a more comprehensive understanding of the different combinations of mechanisms involving transcription, mRNA stability, translation, and protein clearance that operate to regulate these metamorphosis‐associated proteins. © 1993Wiley‐Liss, Inc.
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