BcMF26a and BcMF26b Are Duplicated Polygalacturonase Genes with Divergent Expression Patterns and Functions in Pollen Development and Pollen Tube Formation in Brassica campestris.
BcMF26a and BcMF26b Are Duplicated Polygalacturonase Genes with Divergent Expression Patterns and Functions in Pollen Development and Pollen Tube Formation in Brassica campestris.
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BcMF26a 和 BcMF26b 是重复的多聚半乳糖醛酸酶基因，在油菜花粉发育和花粉管形成中具有不同的表达模式和功能
DOI：
10.1371/journal.pone.0131173
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Cao J
中科院分区：
文献类型：
--
作者：
Lyu M;Yu Y;Jiang J;Song L;Liang Y;Ma Z;Xiong X;Cao J
多聚半乳糖醛酸酶（PG）是一种参与果胶降解的细胞壁水解酶。通过对白菜雄性不育26 a（Brassica campestris Male Fertility 26 a，BcMF 26 b）和两个高度保守的重复PG基因的比较，揭示了它们在表达模式和功能上的不同特点。我们发现这两个基因是At 4g 33440的直向同源基因，它们起源于染色体的节段重复。虽然结构相似，但它们的调控和内含子序列在很大程度上是不同的。QRT-PCR分析表明，BcMF 26 b在白菜几乎所有器官组织中的表达量均高于BcMF 26 a。启动子活性分析表明，在生殖发育阶段，BcMF 26 b启动子在绒毡层、花粉粒和雌蕊中均有活性，而BcMF 26 a启动子仅在雌蕊中有活性。在亚细胞定位实验中，BcMF 26 a和BcMF 26 b蛋白均定位于细胞壁。当这两个基因同时受到抑制时，花粉内壁形成异常，花粉管不能生长或伸长。At 4g 33440基因敲除后，花粉管生长受到抑制。因此，BcMF 26 a/B可能通过调控内壁信息参与花粉壁的构建，BcMF 26 B可能在共抑制转化植株中起主要作用。
Polygalacturonase (PG) is one of the cell wall hydrolytic enzymes involving in pectin degradation. A comparison of two highly conserved duplicated PG genes, namely, Brassica campestris Male Fertility 26a (BcMF26a) and BcMF26b, revealed the different features of their expression patterns and functions. We found that these two genes were orthologous genes of At4g33440, and they originated from a chromosomal segmental duplication. Although structurally similar, their regulatory and intron sequences largely diverged. QRT-PCR analysis showed that the expression level of BcMF26b was higher than that of BcMF26a in almost all the tested organs and tissues in Brassica campestris. Promoter activity analysis showed that, at reproductive development stages, BcMF26b promoter was active in tapetum, pollen grains, and pistils, whereas BcMF26a promoter was only active in pistils. In the subcellular localization experiment, BcMF26a and BcMF26b proteins could be localized to the cell wall. When the two genes were co-inhibited, pollen intine was formed abnormally and pollen tubes could not grow or stretch. Moreover, the knockout mutants of At4g33440 delayed the growth of pollen tubes. Therefore, BcMF26a/b can participate in the construction of pollen wall by modulating intine information and BcMF26b may play a major role in co-inhibiting transformed plants.
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影响因子：
7
作者：
Blanc, G;Hokamp, K;Wolfe, KH
通讯作者：
Wolfe, KH
影响因子：
11.6
作者：
Danilevskaya, ON;Hermon, P;Ananiev, EV
通讯作者：
Ananiev, EV
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
BcMF9 是一种新型多聚半乳糖醛酸酶基因，是甘蓝内壁和外壁形成所必需的
影响因子：
4.2
作者：
Huang, Li;Ye, Yiqun;Cao, Jiashu
通讯作者：
Cao, Jiashu
影响因子：
7.4
作者：
Honys, D;Twell, D
通讯作者：
Twell, D