In Vivo and In Vitro Anti-Arthritic Effects of Cardenolide-Rich and Caffeoylquinic Acid-Rich Fractions of Periploca forrestii.
In Vivo and In Vitro Anti-Arthritic Effects of Cardenolide-Rich and Caffeoylquinic Acid-Rich Fractions of Periploca forrestii.
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富含强心内酯和富含咖啡酰奎宁酸的杠柳组分的体内和体外抗关节炎作用
DOI：
10.3390/molecules23081988
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发表时间：
2018-08-09
期刊：
影响因子：
--
通讯作者：
Li YJ
中科院分区：
文献类型：
--
作者：
Liu T;Wang X;He YL;Wang Y;Dong L;Ma X;Zheng L;Liu CH;Wang GC;Zheng J;Lan YY;Li YJ
林冠病。（P. forrestii）是一种被称为“苗药”的中药，在治疗风湿病、类风湿关节炎（RA）和关节疼痛方面有着悠久的历史。本研究旨在评价富香硬素和富咖啡酰奎宁酸组分（CDLFs和CQAFs）对胶原诱导关节炎（CIA）大鼠的抗关节炎作用，并明确TNF-α对MH7A细胞的治疗作用机制。采用ELISA或其他市售检测试剂盒检测血清类风湿因子（RF）、TNF-α、IL-6、IL-1β、PGE2、NO、SOD和MDA。观察踝关节组织病理变化。采用qRT-PCR法检测MH7A细胞中IL-1β、IL-6、COX-2和iNOS mRNA的表达。Western blot检测MH7A细胞中iNOS、COX-2、p65蛋白的表达以及IκBα、p38、ERK1/2、JNK蛋白的磷酸化水平。结果表明，CDLF和CQAF在不同剂量（125 mg/kg、250 mg/kg和500 mg/kg）下均能抑制CIA大鼠足跖肿胀。组织病理学检查显示，ccdlf和CQAF均能明显减轻CIA大鼠踝关节结构的损伤。血清RF、TNF-α、IL-6、IL-1β、PGE2、NO、MDA降低，血清SOD活性升高。此外，CDLF和CQAF下调IL-1β、IL-6、COX-2、iNOS和p65的表达，抑制TNF-α处理的MH7A细胞中IκBα、p38、ERK1/2和JNK的磷酸化。这些发现表明CDLF和CQAF都具有抗关节炎活性，这可能与它们对NF-κB和MAPK信号通路的抑制作用有关。
Periploca forrestii Schltr. (P. forrestii) is a species used in Traditional Chinese Medicine (TCM) known as “Miao medicine”, and has a long history of use in the treatment of rheumatism, rheumatoid arthritis (RA), and joint pain. The present study aimed to evaluate the anti-arthritis effects of the cardenolide-rich and caffeoylquinic acid-rich fractions (CDLFs and CQAFs) of P. forrestii in collagen-induced arthritic (CIA) rats, and defined the mechanisms of therapeutic action in MH7A cells treated with TNF-α. Serum rheumatoid factor (RF), TNF-α, IL-6, IL-1β, PGE2, NO, SOD, and MDA were determined by ELISA or other commercially assay kits. Histopathological changes in ankle joint tissues were examined. The mRNA expressions of IL-1β, IL-6, COX-2, and iNOS in MH7A cells were measured by qRT-PCR assays. In addition, the expressions of iNOS, COX-2, and p65 proteins, and the phosphorylation of IκBα, p38, ERK1/2, and JNK proteins in MH7A cells were analyzed by Western blot. The results showed that CDLF and CQAF could suppress the paw swelling in CIA rats at different doses (125 mg/kg, 250 mg/kg, and 500 mg/kg). Histopathological examination suggests that the CDLF and CQAF significantly relieved the damage of the structure of the ankle joint in CIA rats. In addition, serum RF, TNF-α, IL-6, IL-1β, PGE2, NO, and MDA were decreased, along with increased activity of serum SOD. Furthermore, CDLF and CQAF downregulated the expressions of IL-1β, IL-6, COX-2, iNOS, and p65, and inhibited the phosphorylation of IκBα, p38, ERK1/2, and JNK in MH7A cells treated with TNF-α. These findings demonstrated that both CDLF and CQAF exhibited anti-arthritic activity, which might be associated with their inhibitory effects on the NF-κB and MAPK signaling pathways.
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影响因子：
56.9
作者：
Johnson, GL;Lapadat, R
通讯作者：
Lapadat, R
影响因子：
1.8
作者：
Hegemann, N;Wondimu, A;Schmidt, MFG
通讯作者：
Schmidt, MFG
影响因子：
5.1
作者：
Loeser RF;Erickson EA;Long DL
通讯作者：
Long DL
金雀异黄素通过调节人滑膜细胞 MH7A 细胞中的活性氧/Akt/核因子 κB 和腺苷单磷酸激活蛋白激酶信号通路来抑制肿瘤坏死因子 α 诱导的炎症
DOI：
10.2147/dddt.s52354
发表时间：
2014
期刊：
Drug design, development and therapy
影响因子：
--
作者：
Li J;Li J;Yue Y;Hu Y;Cheng W;Liu R;Pan X;Zhang P
通讯作者：
Zhang P
影响因子：
0.7
作者：
Foyet HS;Tsala DE;Zogo Essono Bodo JC;Carine AN;Heroyne LT;Oben EK
通讯作者：
Oben EK