High-level erythroid lineage-directed gene expression using globin gene regulatory elements after lentiviral vector-mediated gene transfer into primitive human and murine hematopoietic cells.
High-level erythroid lineage-directed gene expression using globin gene regulatory elements after lentiviral vector-mediated gene transfer into primitive human and murine hematopoietic cells.
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慢病毒载体介导的基因转移至原始人类和小鼠造血细胞后，使用珠蛋白基因调控元件进行高水平红细胞谱系定向基因表达。
DOI：
10.1089/10430340260395866
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Nienhuis,ArthurW
中科院分区：
文献类型：
--
作者：
Hanawa,Hideki;Persons,DerekA;Nienhuis,ArthurW
Lentiviral vectors efficiently transduce primitive human hematopoietic cells and are capable of transferring complex genomes. Vectors were designed with hypersensitive sites containing regulatory elements from theβ-globin locus control region linked to theβ-globin gene promoter to drive expression of the enhanced green fluorescent protein marker to facilitate analysis of the pattern of gene expression in various hematopoietic lineages. Such vectors gave higher level, induced expression in mouse erythroleukemia cells than a previously described vector that utilized an enhancer from the α locus and the ankyrin-1 promoter [Moreau-Gaudry, F., Xia, P., Jiang, G., Perelman, N.P., Bauer, G., Ellis, J., Surinya, K.H., Mavilio, F., Shen, C.K., and Malik, P. (2001). Blood 98, 2664-2672]. The addition ofγ-globin intron sequences further augmented vector expression. Expression was also effectively targeted to the erythroid lineage in cultured human cells from peripheral blood and in mouse red blood cellsin vivo, although lower levels of expression were also observed in other lineages. Thus, these newly described vectors provide a means to achieve high-level gene expression, predominantly in erythroid cells, an outcome that may have potential therapeutic application.
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DOI：
10.1073/pnas.92.15.6728
发表时间：
1995-07
影响因子：
11.1
作者：
M. Sadelain;C.H.J. Wang;M. Antoniou;F. Grosveld;R. Mulligan
通讯作者：
M. Sadelain;C.H.J. Wang;M. Antoniou;F. Grosveld;R. Mulligan
将由自失活人类免疫缺陷病毒 1 型载体转导的人类 CD34 细胞长期植入非肥胖糖尿病/严重联合免疫缺陷小鼠中。
DOI：
10.1089/104303401750214294
发表时间：
2001
期刊：
Human gene therapy.
影响因子：
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作者：
Gatlin,J;Padgett,A;Melkus,MW;Kelly,PF;Garcia,JV
通讯作者：
Garcia,JV
造血谱系定型决策：来自含有 μLCR-βpro-LacZ 作为转基因的转基因小鼠模型的体内证据
DOI：
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发表时间：
2000
期刊：
影响因子：
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作者：
T. Papayannopoulou;G. Priestley;A. Rohde;K. Peterson;B. Nakamoto
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B. Nakamoto
影响因子：
10.5
作者：
Saleque, S;Cameron, S;Orkin, SH
通讯作者：
Orkin, SH
GATA-1 基因上游调控元件内的红细胞和巨核细胞表达的不同序列要求。
DOI：
10.1242/dev.126.12.2799
发表时间：
1999
期刊：
Development (Cambridge, England)
影响因子：
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作者：
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通讯作者：
Orkin,SH