Hemopoietic lineage commitment decisions: in vivo evidence from a transgenic mouse model harboring μLCR-βpro-LacZ as a transgene
Hemopoietic lineage commitment decisions: in vivo evidence from a transgenic mouse model harboring μLCR-βpro-LacZ as a transgene
复制标题
造血谱系定型决策：来自含有 μLCR-βpro-LacZ 作为转基因的转基因小鼠模型的体内证据
DOI：
--
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
B. Nakamoto
中科院分区：
文献类型：
--
作者：
T. Papayannopoulou;G. Priestley;A. Rohde;K. Peterson;B. Nakamoto
A substantial body of published data suggests activation of lineage-specific genes in multipotential hemopoietic cells before their unilineage commitment. Because the behavior and plasticity of cells isolated in vitro away from microenvironmental constraints exercised in vivo may be altered, one wonders whether similar findings can be observed in a physiologic setting in vivo. We used a transgenic mouse model harboring human micro LCR together with beta promoter sequences as a transgene to examine activation of lineage-specific programs in vivo. By using LacZ as a reporter, we had the ability to detect, quantitate, and select live cells with different levels of LacZ activation. We found strong expression of LacZ by X-gal staining in 2 lineages-erythroid and megakaryocytic. Activation in the latter was a novel finding not previously observed when similar transgenes were used. We also found activation of muLCR-betapro at low levels in progenitor cells of granulocytic-macrophagic, erythroid, or megakaryocytic lineage detected by in vitro assays, suggesting activation before commitment to a specific lineage pathway. In particular, the expression of LacZ was graded among progenitors, so that in a proportion of them activation occurred only after commitment to erythroid or megakaryocytic lineage. In addition, we found quantitative reduction in LacZ expression between fetal liver and bone marrow-derived cells, the basis of which is unclear. Collectively our data provide in vivo evidence supporting the view that lineage-specific genes are expressed in a graded fashion in pluripotential cells before their irreversible unilineage commitment. (Blood. 2000;95:1274-1282)
登录
查看更多内容
DOI：
10.1073/pnas.92.21.9647
发表时间：
1995-10-10
影响因子：
11.1
作者：
PAPAYANNOPOULOU, T;CRADDOCK, C;WOLF, NS
通讯作者：
WOLF, NS
影响因子：
56.9
作者：
BERARDI, AC;WANG, AL;SCADDEN, DT
通讯作者：
SCADDEN, DT
影响因子：
10.5
作者：
FRIEDRICH, G;SORIANO, P
通讯作者：
SORIANO, P
DOI：
10.1002/cyto.990120402
发表时间：
1991-01-01
期刊：
CYTOMETRY
影响因子：
--
作者：
FIERING, SN;ROEDERER, M;HERZENBERG, LA
通讯作者：
HERZENBERG, LA
在体外从多能前体细胞产生定型造血集落形成细胞。
影响因子：
20.3
作者：
Nicola,NA;Johnson,GR
通讯作者：
Johnson,GR