Effect of Extracellular Vesicles Derived From Lactobacillus plantarum Q7 on Gut Microbiota and Ulcerative Colitis in Mice.
Effect of Extracellular Vesicles Derived From Lactobacillus plantarum Q7 on Gut Microbiota and Ulcerative Colitis in Mice.
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植物乳杆菌Q7胞外囊泡对小鼠肠道菌群和溃疡性结肠炎的影响
DOI：
10.3389/fimmu.2021.777147
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发表时间：
2021
影响因子：
7.3
通讯作者：
Yi H
中科院分区：
文献类型：
--
作者：
Hao H;Zhang X;Tong L;Liu Q;Liang X;Bu Y;Gong P;Liu T;Zhang L;Xia Y;Ai L;Yi H
益生菌在调节肠道微生物区系、维持肠道内环境平衡等方面发挥着重要作用。益生菌产生的胞外囊泡是机体免疫反应和抗炎作用的潜在介体。然而，益生菌来源的EVS对炎症性肠病(IBD)的抗炎作用及其机制尚不清楚。本研究观察了植物乳杆菌Q7胞外囊泡(Q7-EVS)对C57BL/6J小鼠肠道微生物区系和肠道炎症的影响。结果表明，Q7-EVS可减轻DSS诱导的结肠炎症状，包括结肠缩短、出血和体重减轻。服用Q7-EVS可降低组织学损伤程度。Q7-EVS可显著降低DSS上调的促炎细胞因子IL-6、IL-1β、IL-2和肿瘤坏死因子-α水平(p<；0.05)。16S rRNA测序结果表明，Q7-EVS改善了肠道微生物区系的失调，促进了肠道微生物区系的多样性。观察到促炎细菌(变形杆菌)减少，抗炎细菌(双歧杆菌和小鼠李杆菌科)增加。这些结果表明，Q7-EVS可能通过调节肠道微生物区系来减轻DSS诱导的溃疡性结肠炎。
Probiotics plays an important role in regulating gut microbiota and maintaining intestinal homeostasis. Extracellular vesicles (EVs) derived from probiotics have emerged as potential mediators of host immune response and anti-inflammatory effect. However, the anti-inflammatory effect and mechanism of probiotics derived EVs on inflammatory bowel disease (IBD) remains unclear. In this study, the effect of Lactobacillus plantarum Q7-derived extracellular vesicles (Q7-EVs) on gut microbiota and intestinal inflammation was investigated in C57BL/6J mice. The results showed that Q7-EVs alleviated DSS-induced colitis symptoms, including colon shortening, bleeding, and body weight loss. Consumption of Q7-EVs reduced the degree of histological damage. DSS-upregulated proinflammatory cytokine levels including IL-6, IL-1β, IL-2 and TNF-α were reduced significantly by Q7-EVs (p < 0.05). 16S rRNA sequencing results showed that Q7-EVs improved the dysregulation of gut microbiota and promoted the diversity of gut microbiota. It was observed that the pro-inflammatory bacteria (Proteobacteria) were reduced and the anti-inflammatory bacteria (Bifidobacteria and Muribaculaceae) were increased. These findings indicated that Q7-EVs might alleviate DSS-induced ulcerative colitis by regulating the gut microbiota.
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影响因子：
6.3
作者：
Ishiguro, Y
通讯作者：
Ishiguro, Y
影响因子：
6.1
作者：
Kim, Y. -S.;Choi, E. -J.;Kim, Y. -K.
通讯作者：
Kim, Y. -K.
影响因子：
7.6
作者：
Axelsson, LG;Landstrom, E;Bylund-Fellenius, AC
通讯作者：
Bylund-Fellenius, AC
影响因子：
4.2
作者：
Li M;Lee K;Hsu M;Nau G;Mylonakis E;Ramratnam B
通讯作者：
Ramratnam B
影响因子：
3.9
作者：
Mohamed SS;Abdeltawab NF;Wadie W;Ahmed LA;Ammar RM;Rabini S;Abdel-Aziz H;Khayyal MT
通讯作者：
Khayyal MT