Oral Sensitization of W/Wv Mice with Ovalbumin and Possible Involvement of the Decrease in γδ-T Cells
Oral Sensitization of W/Wv Mice with Ovalbumin and Possible Involvement of the Decrease in γδ-T Cells
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W/Wv 小鼠卵清蛋白口服致敏以及可能与 γδ-T 细胞减少有关
DOI：
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
J. Sawada
中科院分区：
文献类型：
--
作者：
H. Okunuki;R. Teshima;N. Harikai;Shinobu Sakai;H. Akiyama;T. Maitani;J. Sawada
肥大细胞缺陷型WBB 6 F1-W/W（v）小鼠（W/W（v））和同源野生型（+/+）小鼠通过每天以管饲的形式经口施用0.1或1.0mg卵清蛋白（OVA）致敏9周，并且通过腹腔注射OVA诱导主动全身过敏反应（阿萨）。W/W（v）小鼠口服致敏后产生的OVA特异性IgG 1非常高，体外用OVA再刺激的脾细胞产生的IL-4、IL-5和IL-10增加。这些发现表明，Th 2-显性辅助性T细胞活化已经发生。相比之下，OVA特异性IgG 1的产生在+/+小鼠中较低，并且未观察到+/+小鼠的脾细胞产生Th 2型细胞因子的显著增加。通过流式细胞术对派伊尔集合淋巴结中的群体分析显示，抗原刺激后W/W（v）小鼠中CD 11 c（+）细胞的比例略有增加。流式细胞仪分析上皮内淋巴细胞（IEL）的细胞表面标志物显示，W/W（v）小鼠TCR γ δ-T细胞比例极低，尤其是抗原致敏组。W/W（v）小鼠脾细胞中TCR γ δ-T细胞的比例也低于+/+小鼠。综上所述，W/W（v）小鼠不仅是研究食物过敏的诱导机制的良好模型，而且也是研究TCR γ δ-T细胞在食物过敏中的作用的良好模型。
: Mast-cell-deficient WBB6F1-W/W(v) mice (W/W(v)) and congenic wild-type (+/+) mice were sensitized by oral administration of 0.1 or 1.0 mg ovalbumin (OVA) in the form of gavage every day for 9 weeks, and active systemic anaphylaxis (ASA) was induced by intraperitoneal injection of OVA. Production of OVA-specific IgG1 in response to oral sensitization of the W/W(v) mice was very high, and the production of IL-4, IL-5 and IL-10 by splenocytes re-stimulated with OVA in vitro was increased. These findings suggest that Th2-dominant helper T-cell activation had occurred. By contrast, production of OVA-specific IgG1 was low in +/+ mice, and no significant increase in production of Th2-type cytokines by the splenocytes of +/+ mice was observed. Population analysis in Peyer's patches by flow cytometry revealed that the proportion of the CD11c(+) cell in the W/W(v) mice was slightly increased after antigen stimulation. Analysis of the cell surface markers of intraepithelial lymphocytes (IELs) by flow cytometry showed that the proportion of TCRgammadelta-T cells was extremely lower in the W/W(v) mice, especially in the antigen sensitized group. The proportion of TCRgammadelta-T cells in the splenocytes of W/W(v) mice was also lower than in +/+ mice. Taken together, the above findings indicate that W/W(v) mice seems to be a good model not only for studying the induction mechanism of food allergy but for examining the role of TCRgammadelta-T cells in food-induced hypersensitivity.
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影响因子：
10.5
作者：
NOCKA, K;MAJUMDER, S;BESMER, P
通讯作者：
BESMER, P
影响因子：
4.4
作者：
Yong Ke;K. Pearce;J. Lake;H. Ziegler;J. Kapp
通讯作者：
Yong Ke;K. Pearce;J. Lake;H. Ziegler;J. Kapp
由于干细胞因子受体缺乏而导致的年龄依赖性肠道淋巴增殖性疾病：小肠和大肠的参数。
DOI：
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发表时间：
1997
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Laky,K;Lefrançois,L;Puddington,L
通讯作者：
Puddington,L
影响因子：
14.2
作者：
Strait, RT;Morris, SC;Finkelman, FD
通讯作者：
Finkelman, FD
影响因子：
4.4
作者：
Fujihashi, K;Dohi, T;Kiyono, H
通讯作者：
Kiyono, H