In vitro blood cell viability profiling of polymers used in molecular assembly.
In vitro blood cell viability profiling of polymers used in molecular assembly.
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分子组装中使用的聚合物的体外血细胞活力分析。
DOI：
10.1038/s41598-017-10169-5
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发表时间：
2017-08-25
影响因子：
4.6
通讯作者：
Hong J
中科院分区：
文献类型：
--
作者：
Jeong H;Hwang J;Lee H;Hammond PT;Choi J;Hong J
生物相容性聚合物已被广泛应用于微纳米级的分子组装技术，以制备生物医学用途的功能器件。然而，已开发器械的细胞毒性评估倾向于部分关注非特异性细胞或与特定应用相关的细胞。因此，由于毒性取决于细胞的类型和方案，我们并不完全了解聚合物的相对毒性。此外，我们需要确保开发的器械的血细胞生物相容性优于靶细胞生物相容性，因为大多数器械在到达靶区域之前都会接触血液。出于这个问题，我们专注于筛选广泛用于使用人血细胞的层层组装技术的聚合物的细胞毒性。通过溶血、细胞活力和AnnexinV-FITC/PI染色试验，研究了20种聚合物（带正电荷、带负电荷或中性）和10种组合形式的早期细胞毒性。我们通过分子动力学模拟确定了它们对细胞膜的影响，这取决于它们的表面化学性质。此外，通过测量细胞活力来评估LbL组装的纳米膜的毒性。基于这份报告，研究人员可以通过减少可能的细胞毒性来生产更适合药物输送和生物医学应用的纳米膜。
Biocompatible polymers have been extensively applied to molecular assembly techniques on a micro- and nanoscale to miniaturize functional devices for biomedical uses. However, cytotoxic assessments of developed devices are prone to partially focus on non-specific cells or cells associated with the specific applications. Thereby, since toxicity is dependent on the type of cells and protocols, we do not fully understand the relative toxicities of polymers. Additionally, we need to ensure the blood cell biocompatibility of developed devices prior to that of targeted cells because most of the devices contact the blood before reaching the targeted regions. Motivated by this issue, we focused on screening cytotoxicity of polymers widely used for the layer-by-layer assembly technique using human blood cells. Cytotoxicity at the early stage was investigated on twenty types of polymers (positively charged, negatively charged, or neutral) and ten combination forms via hemolysis, cell viability, and AnnexinV-FITC/PI staining assays. We determined their effects on the cell membrane depending on their surface chemistry by molecular dynamics simulations. Furthermore, the toxicity of LbL-assembled nanofilms was assessed by measuring cell viability. Based on this report, researchers can produce nanofilms that are better suited for drug delivery and biomedical applications by reducing the possible cytotoxicity.
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影响因子：
5.5
作者：
Hess, Berk;Kutzner, Carsten;Lindahl, Erik
通讯作者：
Lindahl, Erik
DOI：
10.1021/jp9058966
发表时间：
2009-10-08
期刊：
The journal of physical chemistry. B
影响因子：
--
作者：
Lee H;de Vries AH;Marrink SJ;Pastor RW
通讯作者：
Pastor RW
影响因子：
15
作者：
HUMMERS, WS;OFFEMAN, RE
通讯作者：
OFFEMAN, RE
影响因子：
3.7
作者：
Fu, Kai;Cheng, Qin;Yang, Daichang
通讯作者：
Yang, Daichang
影响因子：
3.3
作者：
Lee, Hwankyu;Larson, Ronald G.
通讯作者：
Larson, Ronald G.