Modifying Dendritic Cell Activation with Plasmonic Nano Vectors.
Modifying Dendritic Cell Activation with Plasmonic Nano Vectors.
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DOI：
10.1038/s41598-017-04459-1
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发表时间：
2017-07-14
影响因子：
4.6
通讯作者：
Biris AS
中科院分区：
文献类型：
--
作者：
Vang KB;Safina I;Darrigues E;Nedosekin D;Nima ZA;Majeed W;Watanabe F;Kannarpady G;Kore RA;Casciano D;Zharov VP;Griffin RJ;Dings RPM;Biris AS
树突状细胞（DC）可以获得、加工并将抗原呈递给T细胞以诱导免疫应答。因此，将癌症抗原靶向DC以引起针对癌症的免疫反应是纳米医学的一个新兴领域，它有可能重新定义某些癌症的治疗方式。使用等离子体活性的银涂覆的金纳米棒（此后称为等离子体纳米载体（PNV））作为DC肿瘤疫苗的潜在载体之前尚未提出。有效的载体必须能够被DC吞噬，呈现低毒性，并诱导DC成熟-免疫应答的早期指示。当我们用PNV处理DC时，我们发现DC的细胞活力不受影响，高达200 μg/ml。此外，PNV与DC相关联，因为它们被吞噬，并且它们被发现驻留在细胞内区室如内体内。更重要的是，PNV能够诱导指示DC活化和成熟的表面标志物，即CD 40、CD 86和MHC II类的表达。这些结果提供了第一个证据表明，PNV是有前途的载体DC为基础的疫苗，并保证进一步调查的临床使用。
Dendritic cells (DCs) can acquire, process, and present antigens to T-cells to induce an immune response. For this reason, targeting cancer antigens to DCs in order to cause an immune response against cancer is an emerging area of nanomedicine that has the potential to redefine the way certain cancers are treated. The use of plasmonically active silver-coated gold nanorods (henceforth referred to as plasmonic nano vectors (PNVs)) as potential carriers for DC tumor vaccines has not been presented before. Effective carriers must be able to be phagocytized by DCs, present low toxicity, and induce the maturation of DCs—an early indication of an immune response. When we treated DCs with the PNVs, we found that the cell viability of DCs was unaffected, up to 200 μg/ml. Additionally, the PNVs associated with the DCs as they were phagocytized and they were found to reside within intracellular compartments such as endosomes. More importantly, the PNVs were able to induce expression of surface markers indicative of DC activation and maturation, i.e. CD40, CD86, and MHC class II. These results provide the first evidence that PNVs are promising carriers for DC-based vaccines and warrant further investigating for clinical use.
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影响因子：
4.6
作者：
Damo M;Wilson DS;Simeoni E;Hubbell JA
通讯作者：
Hubbell JA
影响因子：
5.2
作者：
Galanzha EI;Zharov VP
通讯作者：
Zharov VP
影响因子：
10.8
作者：
Batista de Faria, Paula Cristina;dos Santos, Luara Isabela;Gazzinelli, Ricardo Tostes
通讯作者：
Gazzinelli, Ricardo Tostes
影响因子：
4.4
作者：
Carter, Robert W.;Thompson, Clare;Tough, David F.
通讯作者：
Tough, David F.
影响因子：
38.3
作者：
Jiang, Wen;Kim, Betty Y. S.;Chan, Warren C. W.
通讯作者：
Chan, Warren C. W.