A bestrophin-like protein modulates the proton motive force across the thylakoid membrane in Arabidopsis.
A bestrophin-like protein modulates the proton motive force across the thylakoid membrane in Arabidopsis.
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DOI：
10.1111/jipb.12475
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发表时间：
2016-10
影响因子：
11.4
通讯作者：
Peng L
中科院分区：
文献类型：
--
作者：
Duan Z;Kong F;Zhang L;Li W;Zhang J;Peng L
在光合作用过程中，光合作用电子传递产生穿过类囊体膜的质子动力(PMF)，用于通过叶绿体中的ATP合成酶生物合成ATP。PMF由一个电势(ΔΨ)和一个渗透成分(Δ，pH)组成。叶绿体中这些组分之间的分配受到严格的调控，以应对环境的波动。然而，我们对PMF分配的分子机制的了解是有限的。在这里，我们报告了一种类Bestrophin蛋白(AtBest)，它对PMF的分割至关重要。虽然Δ的pH分量在最好的情况下略有降低，但该突变体的ΔΨ分量比野生型的要大得多，导致非光化学猝灭在光照下的激活效率较低，以及从弱光到强光的转变。虽然温室中最好的突变体没有观察到明显的表型，但在田间生长时，该突变体表现出比野生型更强的光抑制。AtBest属于Bestrophin家族蛋白，被认为具有氯(Cl−)通道的功能。因此，我们的发现揭示了高等植物类囊体膜上离子转运和动态平衡所需的重要的氯−通道。这些过程对于在波动的环境条件下微调光合作用是必不可少的。
During photosynthesis, photosynthetic electron transport generates a proton motive force (pmf) across the thylakoid membrane, which is used for ATP biosynthesis via ATP synthase in the chloroplast. The pmf is composed of an electric potential (ΔΨ) and an osmotic component (ΔpH). Partitioning between these components in chloroplasts is strictly regulated in response to fluctuating environments. However, our knowledge of the molecular mechanisms that regulate pmf partitioning is limited. Here, we report a bestrophin‐like protein (AtBest), which is critical for pmf partitioning. While the ΔpH component was slightly reduced in atbest, the ΔΨ component was much greater in this mutant than in the wild type, resulting in less efficient activation of nonphotochemical quenching (NPQ) upon both illumination and a shift from low light to high light. Although no visible phenotype was observed in the atbest mutant in the greenhouse, this mutant exhibited stronger photoinhibition than the wild type when grown in the field. AtBest belongs to the bestrophin family proteins, which are believed to function as chloride (Cl−) channels. Thus, our findings reveal an important Cl− channel required for ion transport and homeostasis across the thylakoid membrane in higher plants. These processes are essential for fine‐tuning photosynthesis under fluctuating environmental conditions.
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DOI：
10.1085/jgp.200609581
发表时间：
2006-09
期刊：
The Journal of general physiology
影响因子：
--
作者：
Chien LT;Zhang ZR;Hartzell HC
通讯作者：
Hartzell HC
拟南芥氯通道基因家族的克隆和分子分析
影响因子：
5.2
作者：
Lv, Qun-dan;Tang, Ren-jie;Zhang, Hong-xia
通讯作者：
Zhang, Hong-xia
影响因子：
6.9
作者：
Marmagne, Anne;Vinauger-Douard, Marion;Ephritikhine, Genevieve
通讯作者：
Ephritikhine, Genevieve
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
56.9
作者：
Carraretto, Luca;Formentin, Elide;Szabo, Ildiko
通讯作者：
Szabo, Ildiko