Peripheral nerve injury induces a transitory microglial reaction in the rat infralimbic cortex
Peripheral nerve injury induces a transitory microglial reaction in the rat infralimbic cortex
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周围神经损伤诱导大鼠边缘下皮质短暂性小胶质细胞反应
DOI：
10.1016/j.neulet.2017.06.037
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发表时间：
2017
影响因子：
2.5
通讯作者：
R. Gosselin
中科院分区：
文献类型：
--
作者：
P. C. S. Chung;T. Panigada;Romain Cardis;I. Decosterd;R. Gosselin
不可否认的证据表明，脊髓中的小胶质细胞在外周损伤后发生明显的反应。然而，只有很少的研究调查了周围神经损伤后大脑区域可能的短期和长期小胶质细胞反应及其种间特异性。在本研究中，我们检测了小鼠和大鼠在坐骨神经幸免神经损伤（SNI）后7天和42天的前额皮质亚区小胶质细胞。我们发现，大鼠损伤后7天，双侧边缘下皮层小胶质细胞密度增加（sham vs. SNI, n = 5：同侧中位数增加35.4%,p = 0.0317；对侧中位数增加24.9%,p = 0.0079），其他研究区域，即前扣带、边缘前和颗粒岛皮质未见明显变化。在小鼠中，无论是使用Iba-1免疫染色还是使用CX3CR1-eGFP动物，在两个时间点的任何区域都没有发现可观察到的差异。我们的研究结果表明，周围神经损伤后，前额叶皮层发生了短暂的、物种特异性的、高度区域化的小胶质细胞反应。
Undeniable evidence shows that microglia in the spinal cord undergo marked reactions following peripheral injuries. However, only rare studies have investigated the possible short and long term microglial reaction in brain regions following peripheral nerve injury and its interspecies specificities. In the present study we examined microglia in subdivisions of the prefrontal cortex in mice and rats, 7 days and 42 days after spared nerve injury (SNI) of the sciatic nerve. We show that a bilateral increase of microglial density takes place in the infralimbic cortex in rats 7 days post-injury (sham vs. SNI, n = 5: ipsilateral 35.4% increase of the median, p = 0.0317; contralateral 24.9% increase of the median, p = 0.0079), without any detectable change in the other investigated regions, namely the anterior cingulate, prelimbic and agranular insular cortices. In mice, no observable difference could be found in any region at both time points, neither using Iba-1 immunostaining nor with CX3CR1-eGFP animals. Our results indicate that a transitory, species-specific and highly regionalized microglial reaction takes place in the prefrontal cortex following peripheral nerve injury.
DOI：
10.1101/lm.035501.114
发表时间：
2014-09
期刊：
Learning & memory (Cold Spring Harbor, N.Y.)
影响因子：
--
作者：
Barker JM;Taylor JR;Chandler LJ
通讯作者：
Chandler LJ