Neurotrophin-4/5 is implicated in the enhancement of axon regeneration produced by treadmill training following peripheral nerve injury.
Neurotrophin-4/5 is implicated in the enhancement of axon regeneration produced by treadmill training following peripheral nerve injury.
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神经营养蛋白4/5与周围神经损伤后跑步机训练产生的轴突再生有关。
DOI：
10.1111/j.1460-9568.2011.07724.x
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发表时间：
2011-06
期刊：
影响因子：
--
通讯作者：
Sabatier MJ
中科院分区：
文献类型：
--
作者：
English AW;Cucoranu D;Mulligan A;Rodriguez JA;Sabatier MJ
本实验研究了神经营养素-4/5在小鼠跑台训练后促进周围神经轴突再生中的作用。对THY-1-YFP小鼠H系动物的腓总神经进行了切断，并用非荧光小鼠的神经移植物进行了手术修复。取整组神经制作光学切片，测量荧光再生轴突的长度。将每天以中等速度(10m/min)进行一小时跑步机训练的小鼠的测量值与未训练(对照组)小鼠的测量值进行比较。适度的跑步机训练导致在一周、两周和四周的存活时间中，荧光轴突的轮廓几乎是对照组的两倍。用神经营养素-4/5基因敲除小鼠的移植物或经反复冻融脱细胞的移植物修复野生型小鼠切断的神经，也有类似的促进再生作用。无论用于修复切断的神经的移植物的性质如何，在神经营养素-4/5基因敲除的小鼠中，跑步机训练没有产生任何增强作用。跑步机训练对切断的周围神经轴突再生的影响不依赖于切断神经远段的变化，而是依赖于近端神经节段细胞NT-4/5表达的变化而促进轴突再生。
The role of neurotrophin-4/5 in the enhancement of axon regeneration in peripheral nerves produced by treadmill training was studied in mice. Common fibular nerves of animals of the H strain of thy-1-YFP mice, in which a subset of axons in peripheral nerves is marked by the presence of yellow fluorescent protein, were cut and surgically repaired using nerve grafts from non-fluorescent mice. Lengths of profiles of fluorescent regenerating axons were measured using optical sections made through whole mounts of harvested nerves. Measurements from mice that had undergone one hour of daily treadmill training at modest speed (10 m/min) were compared to those of untrained (control) mice. Modest treadmill training resulted in fluorescent axon profiles that were nearly twice as long as controls at one, two and four week survival times. Similar enhanced regeneration was found when cut nerves of wild type mice were repaired with grafts from neurotrophin-4/5 knockout mice or grafts made acellular by repeated freezing/thawing. No enhancement was produced by treadmill training in neurotrophin-4/5 knockout mice, irrespective of the nature of the graft used to repair the cut nerve. Much as had been observed previously for the effects of brief electrical stimulation, the effects of treadmill training on axon regeneration in cut peripheral nerves are independent of changes produced in the distal segment of the cut nerve and depend on the promotion of axon regeneration by changes in NT-4/5 expression by cells in the proximal nerve segment.
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影响因子：
12.7
作者：
ANDERSON, PN;NADIM, W;TURMAINE, M
通讯作者：
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