Vibrio cholerae's mysterious Seventh Pandemic island (VSP-II) encodes novel Zur-regulated zinc starvation genes involved in chemotaxis and cell congregation.
Vibrio cholerae's mysterious Seventh Pandemic island (VSP-II) encodes novel Zur-regulated zinc starvation genes involved in chemotaxis and cell congregation.
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霍乱弧菌的神秘的第七次大流行岛（VSP-II）编码新的细菌调节锌饥饿基因参与趋化性和细胞聚集。
DOI：
10.1371/journal.pgen.1009624
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发表时间：
2021-06
期刊：
影响因子：
4.5
通讯作者：
Dörr T
中科院分区：
文献类型：
--
作者：
Murphy SG;Johnson BA;Ledoux CM;Dörr T
霍乱弧菌是霍乱的病原体，霍乱是一种臭名昭著的腹泻病，通常通过受污染的饮用水传播。当前的大流行病原体，El Tor 生物型，经历了几次基因变化，包括水平获得两个基因组岛（VSP-I 和 VSP-II）。 VSP 的存在与流行病密切相关；然而，这些岛对霍乱弧菌生命周期的贡献，特别是 26 kb VSP-II，仍然知之甚少。 VSP-II 编码的基因在标准实验室条件下不表达，这表明它们的诱导需要来自宿主或环境的未知信号。细菌在宿主和环境条件下遇到的一个信号是金属限制。在体外研究霍乱弧菌的锌饥饿反应时，我们注意到，当在营养不良的培养基中生长时，组成型表达锌饥饿基因（Δzur）的突变体聚集在培养管的底部。通过转座子诱变，我们发现鞭毛运动、趋化性和 VSP-II 编码基因是聚集所必需的。 VSP-II 基因编码 AraC 样转录激活因子 (VerA) 和甲基接受趋化蛋白 (AerB)。使用 RNA-seq 和 lacZ 转录报告基因，我们证明 VerA 是一种新的 Zur 靶标，也是附近 AerB 化学感受器的激活剂。 AerB 与趋化系统相互作用，驱动依赖氧气的聚集和能量出租车。重要的是，这项工作提出了 VSP-II、缺锌环境和能源出租车之间的功能联系，从而深入了解 VSP-II 在金属限制宿主或水生水库中的作用。第七次大流行弧菌岛是由埃尔托大流行菌株横向获得的，但其在致病性或环境持久性中的作用尚不清楚。 VSP-II 研究的一个主要障碍是缺乏有利于其表达的刺激。我们发现锌饥饿会诱导岛成分的表达，并描述了激活 VSP-II 编码的能量趋向受体的转录网络。重要的是，能量出租车可能使霍乱弧菌能够找到更有利的微环境，可能在肠道或环境沉积物的缺氧部分定殖。
Vibrio cholerae is the causative agent of cholera, a notorious diarrheal disease that is typically transmitted via contaminated drinking water. The current pandemic agent, the El Tor biotype, has undergone several genetic changes that include horizontal acquisition of two genomic islands (VSP-I and VSP-II). VSP presence strongly correlates with pandemicity; however, the contribution of these islands to V. cholerae’s life cycle, particularly the 26-kb VSP-II, remains poorly understood. VSP-II-encoded genes are not expressed under standard laboratory conditions, suggesting that their induction requires an unknown signal from the host or environment. One signal that bacteria encounter under both host and environmental conditions is metal limitation. While studying V. cholerae’s zinc-starvation response in vitro, we noticed that a mutant constitutively expressing zinc starvation genes (Δzur) congregates at the bottom of a culture tube when grown in a nutrient-poor medium. Using transposon mutagenesis, we found that flagellar motility, chemotaxis, and VSP-II encoded genes were required for congregation. The VSP-II genes encode an AraC-like transcriptional activator (VerA) and a methyl-accepting chemotaxis protein (AerB). Using RNA-seq and lacZ transcriptional reporters, we show that VerA is a novel Zur target and an activator of the nearby AerB chemoreceptor. AerB interfaces with the chemotaxis system to drive oxygen-dependent congregation and energy taxis. Importantly, this work suggests a functional link between VSP-II, zinc-starved environments, and energy taxis, yielding insights into the role of VSP-II in a metal-limited host or aquatic reservoir. The Vibrio Seventh Pandemic island was horizontally acquired by the El Tor pandemic strain, but its role in pathogenicity or environmental persistence is unknown. A major barrier to VSP-II study was the lack of stimuli favoring its expression. We show that zinc starvation induces expression of island components and describe a transcriptional network that activates a VSP-II encoded energy taxis receptor. Importantly, energy taxis may enable V. cholerae to locate more favorable microenvironments, possibly to colonize anoxic portions of the gut or environmental sediments.
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影响因子：
64.8
作者：
Broza, M;Halpern, M
通讯作者：
Halpern, M
DOI：
10.1073/pnas.0308052101
发表时间：
2004-04-06
影响因子：
11.1
作者：
Butler, SM;Camilli, A
通讯作者：
Camilli, A
影响因子：
6.4
作者：
Dalia AB;Lazinski DW;Camilli A
通讯作者：
Camilli A
影响因子：
3.1
作者：
Campoy, S;Jara, M;Barbé, J
通讯作者：
Barbé, J
DOI：
10.1073/pnas.0609359104
发表时间：
2007-02-20
影响因子：
11.1
作者：
Alexander, Roger P.;Zhulin, Igor B.
通讯作者：
Zhulin, Igor B.