Assessment of 3D MINFLUX data for quantitative structural biology in cells revisited

Assessment of 3D MINFLUX data for quantitative structural biology in cells revisited
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重新审视细胞定量结构生物学的 3D MINFLUX 数据评估

DOI:
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发表时间:
2022
期刊:
bioRxiv
影响因子:
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通讯作者:
Stefan W. Hell
Stefan W. Hell
中科院分区:
--
文献类型:
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作者:
K. Gwosch;F. Balzarotti;Jasmin K. Pape;P. Hoess;J. Ellenberg;J. Ries;U. Matti;R. Schmidt;S. Sahl;Stefan W. Hell

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Prakash and Curd provide a re-analysis1 of individual datasets taken from our report2 demonstrating MINFLUX 3D imaging in cells. Their evaluation confirms the unique localization precision provided by MINFLUX2,3 featuring a standard deviation of σ = 1-3 nm. We appreciate their confirmation and also welcome the opportunity to clarify their remaining points. The hitherto almost unconceivable 3D localization precision attained by MINFLUX is likely to hold the key to an all-optical dynamical structural biology.
评估细胞定量结构生物学的 3D MINFLUX 数据
DOI: 10.1038/s41592-022-01694-x
发表时间: 2022
期刊: Nature Methods
影响因子: 48
作者:
Prakash K
通讯作者: Prakash K
DOI: 10.1242/jcs.156620
发表时间: 2014-10-15
影响因子: 4
作者:
Loeschberger, Anna;Franke, Christian;Sauer, Markus
通讯作者: Sauer, Markus