The mudskipper, Periophthalmodon schlosseri, actively transports[Formula: see text] against a concentration gradient.

The mudskipper, Periophthalmodon schlosseri, actively transports[Formula: see text] against a concentration gradient.
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弹涂鱼(Periophthalmodon schlosseri)逆浓度梯度主动运输[分子式:见正文]。

DOI:
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发表时间:
1999
期刊:
American Journal of Physiology. Regulatory Integrative and Comparative Physiology
影响因子:
--
通讯作者:
Y. Ip
Y. Ip
中科院分区:
--
文献类型:
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作者:
D. Randall;J. Wilson;K. Peng;T. Kok;S. Kuah;S. Chew;T. Lam;Y. Ip

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当暴露在8和30 mM的NH4Cl中时,眼周鱼可以保持氨的排泄率和组织中的低水平氨,但当该鱼暴露在50%的海水中时,组织中的氨水平会上升。由于跨皮细胞的电位不足以维持血液和水之间的浓度梯度,在这种情况下,氨的排泄似乎是活跃的。鳃的Na+-K+-ATPase活性非常高,可以被生理水平的[公式:参见文本]而不是K+激活。在外部介质中加入哇巴因和阿米洛利可抑制鱼对浓度梯度的排氨。提示Na+-K+-ATPase和安娜+/H+交换器可能参与了氨跨鳃的主动排泄。斯氏假单胞菌这种主动排氨的独特能力与其鳃的特殊结构有关,使鱼在暴露在空气中或在洞穴中继续排泄氨。
Periophthalmodon schlosseri can maintain ammonia excretion rates and low levels of ammonia in its tissues when exposed to 8 and 30 mM NH4Cl, but tissue ammonia levels rise when the fish is exposed to 100 mM NH4Cl in 50% seawater. Because the transepithelial potential is not high enough to maintain the[Formula: see text] concentration gradient between blood and water, ammonia excretion under such a condition would appear to be active. Branchial Na+-K+-ATPase activity is very high and can be activated by physiological levels of[Formula: see text] instead of K+. Ammonia excretion by the fish against a concentration gradient is inhibited by the addition of ouabain and amiloride to the external medium. It is concluded that Na+-K+-ATPase and an Na+/H+exchanger may be involved in the active excretion of ammonia across the gills. This unique ability of P. schlosseri to actively excrete ammonia is related to the special structure of its gills and allows the fish to continue to excrete ammonia while air exposed or in its burrow.
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