The involvement of RhoA and Wnt-5a in the tumorigenesis and progression of ovarian epithelial carcinoma.
The involvement of RhoA and Wnt-5a in the tumorigenesis and progression of ovarian epithelial carcinoma.
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RhoA和Wnt-5a参与卵巢上皮癌的发生和进展
DOI：
10.3390/ijms141224187
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发表时间：
2013-12-12
影响因子：
5.6
通讯作者：
Zhao Y
中科院分区：
文献类型：
--
作者：
Chen S;Wang J;Gou WF;Xiu YL;Zheng HC;Zong ZH;Takano Y;Zhao Y
Ras同源基因家族成员A （RhoA）参与了wnt -5a诱导的胃癌和乳腺癌细胞的迁移。我们研究了RhoA和Wnt-5a在卵巢癌中的作用。测定正常输卵管上皮、良性肿瘤、原发性卵巢癌和转移性网膜中RhoA和Wnt-5a mRNA和蛋白的表达。利用sirna敲低OVCAR3卵巢癌细胞中的RhoA或Wnt-5a，检测细胞表型和相关分子的表达。RhoA和Wnt-5a mRNA和蛋白在转移网膜中的表达明显高于卵巢癌、良性肿瘤和正常输卵管上皮（p < 0.05），并与卵巢癌的分化和FIGO分期（I/II期vs. III/IV期）呈正相关（p < 0.05）。卵巢癌中RhoA与Wnt-5a表达呈正相关（p = 0.001, R2 = 0.1669）。RhoA或Wnt-5a敲除下调RhoA和Wnt-5a的表达；细胞增殖减少；促进G1阻滞和细胞凋亡；抑制板足形成、细胞迁移和侵袭；降低PI3K、Akt、p70S6k、Bcl-xL、survivin和VEGF mRNA或蛋白的表达。这是首次证明RhoA和Wnt-5a与卵巢癌发生和细胞凋亡抑制有关；RhoA与Wnt-5a表达可能呈正相关。RhoA是卵巢癌中潜在的肿瘤发生、分化和进展的生物标志物。
Ras homolog gene family member A (RhoA) is involved in Wnt-5a–induced migration of gastric and breast cancer cells. We investigated the roles of RhoA and Wnt-5a in ovarian carcinoma. RhoA and Wnt-5a mRNA and protein expression in normal fallopian tube epithelium, benign tumors, primary ovarian carcinomas, and metastatic omentum were quantified. RhoA or Wnt-5a was knocked down in OVCAR3 ovarian carcinoma cells using siRNAs and cell phenotype and expression of relevant molecules were assayed. RhoA and Wnt-5a mRNA and protein expression were found to be significantly higher in metastatic omentum than in ovarian carcinomas, benign tumors, and normal fallopian tube epithelium (p < 0.05), and positively associated with differentiation and FIGO staging (stage I/II vs. stage III/IV) in ovarian carcinoma (p < 0.05). RhoA and Wnt-5a expression were positively correlated in ovarian carcinoma (p = 0.001, R2 = 0.1669). RhoA or Wnt-5a knockdown downregulated RhoA and Wnt-5a expression; reduced cell proliferation; promoted G1 arrest and apoptosis; suppressed lamellipodia formation, cell migration, and invasion; and reduced PI3K, Akt, p70S6k, Bcl-xL, survivin, and VEGF mRNA or protein expression. This is the first demonstration that RhoA and Wnt-5a are associated with ovarian carcinogenesis and apoptosis inhibition; there might be positive correlation between RhoA and Wnt-5a expression. RhoA is a potential tumorigenesis, differentiation, and progression biomarker in ovarian carcinoma.
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影响因子：
4.1
作者：
Kurayoshi, Manabu;Yamamoto, Hideki;Kikuchi, Akira
通讯作者：
Kikuchi, Akira
Wnt5a 作为患者不良临床结果的预测因子和卵巢癌化疗耐药的调节因子
DOI：
10.1097/igc.0b013e31820aaadb
发表时间：
2011-02-01
影响因子：
4.8
作者：
Peng, Chunjie;Zhang, Xiaolei;Zheng, Jianhua
通讯作者：
Zheng, Jianhua
影响因子：
4.8
作者：
Denoyelle, C;Albanese, P;Soria, C
通讯作者：
Soria, C
影响因子：
3.3
作者：
Peacock, Justin G.;Miller, Ann L.;Koleske, Anthony J.
通讯作者：
Koleske, Anthony J.
影响因子：
4.2
作者：
Badiglian Filho, Levon;Fujiyama Oshima, Celina Tizuko;Goncalves, Wagner Jose
通讯作者：
Goncalves, Wagner Jose