Effect of Paris saponin I on radiosensitivity in a gefitinib-resistant lung adenocarcinoma cell line.
Effect of Paris saponin I on radiosensitivity in a gefitinib-resistant lung adenocarcinoma cell line.
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重楼皂苷 I 对吉非替尼耐药肺腺癌细胞系放射敏感性的影响
DOI：
10.3892/ol.2014.2020
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发表时间：
2014-06
期刊：
影响因子：
2.9
通讯作者：
Ma S
中科院分区：
文献类型：
--
作者：
Jiang H;Zhao P;Feng J;Su D;Ma S
先前的研究已经发现，巴黎皂苷I （PSI）具有广泛的药理活性，包括对许多恶性肿瘤（如非小细胞肺癌）的细胞毒活性。本研究旨在探讨PSI治疗对吉非替尼耐药肺腺癌细胞PC-9-ZD的放射增敏作用及其可能的机制。采用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四唑测定法测定PSI的生长抑制作用。通过克隆实验确定PSI对PC-9-ZD细胞系的放射增敏作用。使用单命中多目标模型绘制生存曲线并计算致敏增强比。流式细胞术分析细胞周期，荧光素-异硫氰酸-膜联蛋白V/碘化丙啶染色和Hoechst染色分析细胞凋亡。western blotting检测蛋白表达水平。经PSI处理的PC-9-ZD细胞系的增殖明显减少。PSI增强PC-9-ZD细胞的放射敏感性，增敏增强比为1.77。此外，PSI诱导辐照PC-9-ZD细胞G2/M阻滞和凋亡。值得注意的是，在PSI治疗下，b细胞淋巴瘤2 （Bcl-2）下调，caspase-3、Bcl-2样蛋白4 （Bax）和细胞周期蛋白依赖性激酶抑制剂1 （P21waf1/cip1）上调。本研究表明，PSI治疗在体外对吉非替尼耐药PC-9-ZD细胞表现出强大的放射敏感性。这种放射敏感性与细胞周期阻滞在G2/M期有关，并通过caspase-3、Bax和P21waf1/cip1的增加以及Bcl-2产生的减少而导致细胞凋亡。
Previous studies have observed that Paris saponin I (PSI) exerts a wide range of pharmacological activities, including cytotoxic activity against a number of malignancies, such as non-small cell lung cancers. The present study aimed to investigate the radiosensitization of PSI treatment on a gefitinib-resistant lung adenocarcinoma cell line, PC-9-ZD, and its possible mechanism. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay was used to determine the growth inhibition effect of PSI. A clonogenic assay was performed to determine the radiosensitizing effect of PSI treatment on the PC-9-ZD cell line. A single-hit multi-target model was used to plot survival curves and calculate sensitizing enhancement ratios. The cell cycle was analyzed by flow cytometry and cell apoptosis was analyzed with fluorescein-isothiocyanate-Annexin V/propidium iodide and Hoechst staining. The expression levels of the proteins were detected by western blotting. There was a significant reduction observed in the proliferation of the PC-9-ZD cell lines that were treated with PSI. PSI enhanced the radiosensitivity of the PC-9-ZD cells with a sensitization enhancement ratio of 1.77. Furthermore, PSI induced G2/M arrest and apoptosis of the irradiated PC-9-ZD cells. Notably, B-cell lymphoma 2 (Bcl-2) was downregulated, and caspase-3, Bcl-2-like protein 4 (Bax) and cyclin-dependent kinase inhibitor 1 (P21waf1/cip1) were upregulated by the PSI treatment. The present study showed that PSI treatment exhibited potent radiosensitivity against gefitinib-resistant PC-9-ZD cells in vitro. This radiosensitivity was associated with cell cycle arrest at the G2/M phase, and apoptosis via an increase in caspase-3, Bax and P21waf1/cip1 as well as a decrease in Bcl-2 production.
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影响因子：
9.7
作者：
Cheung, JYN;Ong, RCY;Kong, SK
通讯作者：
Kong, SK
影响因子：
4.3
作者：
Tang, J. J.;Shen, C.;Lu, Y. J.
通讯作者：
Lu, Y. J.
影响因子：
3.4
作者：
Zha, Lin;Qiao, Tiankui;Lei, Linjie
通讯作者：
Lei, Linjie
影响因子：
2.3
作者：
Ji, Yuan;Ma, Sheng-lin;Lu, Yan-Jun
通讯作者：
Lu, Yan-Jun
DOI：
10.1016/j.bbamcr.2008.01.008
发表时间：
2008-05-01
影响因子：
5.1
作者：
Ma, Shenglin;Tang, Juanjuan;Lu, Yanjun
通讯作者：
Lu, Yanjun