Detection of retinitis pigmentosa by differential interference contrast microscopy.
Detection of retinitis pigmentosa by differential interference contrast microscopy.
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DOI：
10.1371/journal.pone.0097170
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Kim JH
中科院分区：
文献类型：
--
作者：
Oh J;Kim SH;Kim YJ;Lee H;Cho JH;Cho YH;Kim CK;Lee TJ;Lee S;Park KH;Yu HG;Lee HJ;Jun SC;Kim JH
微分干涉对比显微镜的目的是图像未染色和透明的标本，通过提高对比度所产生的Nomarski棱镜影响的光程差。视网膜色素变性是最常见的遗传性视网膜疾病之一，其特征在于光感受器的进行性丧失。在这项研究中，微分干涉相差显微镜作为一个新的和简单的应用程序进行评估，观察视网膜感光层和视网膜色素变性的诊断和监测。将皇家外科医学院大鼠和视网膜变性小鼠（两者均为已建立的疾病动物模型）的视网膜组织制备为代表自疾病发生以来不同经过时间的平片和组织切片。光镜下可见视网膜色素变性早期光感受器层的镶嵌结构不规则，部分塌陷，晚期光感受器层的镶嵌结构无定形。同样，组织学切片显示早期感光层变薄，晚期外核层丢失。为了计算和比较正常和早期视网膜色素变性组织中的光感受器的数量，使用形态学重建方法，使用数值计算语言MATLAB设计自动细胞计数程序，应用于微分干涉对比显微图像。细胞数量显著减少，平均而言，皇家外科学院大鼠从282个细胞减少到143个细胞，视网膜变性小鼠从255个细胞减少到170个细胞。我们成功地证明了微分干涉相差显微镜技术的诊断和监测RP的应用潜力。
Differential interference contrast microscopy is designed to image unstained and transparent specimens by enhancing the contrast resulting from the Nomarski prism-effected optical path difference. Retinitis pigmentosa, one of the most common inherited retinal diseases, is characterized by progressive loss of photoreceptors. In this study, Differential interference contrast microscopy was evaluated as a new and simple application for observation of the retinal photoreceptor layer and retinitis pigmentosa diagnostics and monitoring. Retinal tissues of Royal College of Surgeons rats and retinal-degeneration mice, both well-established animal models for the disease, were prepared as flatmounts and histological sections representing different elapsed times since the occurrence of the disease. Under the microscopy, the retinal flatmounts showed that the mosaic pattern of the photoreceptor layer was irregular and partly collapsed at the early stage of retinitis pigmentosa, and, by the advanced stage, amorphous. The histological sections, similarly, showed thinning of the photoreceptor layer at the early stage and loss of the outer nuclear layer by the advanced stage. To count and compare the number of photoreceptors in the normal and early-retinitis pigmentosa-stage tissues, an automated cell-counting program designed with MATLAB, a numerical computing language, using a morphological reconstruction method, was applied to the differential interference contrast microscopic images. The number of cells significantly decreased, on average, from 282 to 143 cells for the Royal College of Surgeons rats and from 255 to 170 for the retinal-degeneration mouse. We successfully demonstrated the potential of the differential interference contrast microscopy technique’s application to the diagnosis and monitoring of RP.
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影响因子：
4
作者：
Zarubiieva, Iuliia;Hwang, Gi Byoung;Kim, Jae Hun
通讯作者：
Kim, Jae Hun
DOI：
10.1109/isbi.2009.5193169
发表时间：
2009-06
期刊：
Proceedings. IEEE International Symposium on Biomedical Imaging
影响因子：
--
作者：
Kothari S;Chaudry Q;Wang MD
通讯作者：
Wang MD
DOI：
10.1073/pnas.0605790103
发表时间：
2006-11-14
影响因子：
11.1
作者：
Cheng, Haiying;Nair, Govind;Duong, Timothy Q.
通讯作者：
Duong, Timothy Q.
影响因子：
4.2
作者：
Fariss, RN;Li, ZY;Milam, AH
通讯作者：
Milam, AH
影响因子：
3.5
作者：
D'Cruz, PM;Yasumura, D;Vollrath, D
通讯作者：
Vollrath, D