Evidence for reduced CTX-M carriage in cattle-associated Escherichia coli at low temperatures and on publicly accessible farmland: implications for surveillance and potential for farm-to-human transmission
Evidence for reduced CTX-M carriage in cattle-associated Escherichia coli at low temperatures and on publicly accessible farmland: implications for surveillance and potential for farm-to-human transmission
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低温下和公共农田中与牛相关的大肠杆菌中 CTX-M 携带减少的证据：对监测的影响和农场向人类传播的可能性
DOI：
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
K. Reyher
中科院分区：
文献类型：
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作者：
Hannah Schubert;J. Findlay;Katy Morley;E. Puddy;Robert E. Arbon;V. Gould;O. Mounsey;Madeleine H. R. Evans;G. Rees;D. Barrett;K. Turner;T. Cogan;M. Avison;K. Reyher
对于农场中存在的具有人畜共患病潜力的物种（例如，CTX-M β-内酰胺酶阳性大肠杆菌）中至关重要的抗菌药物耐药性驱动因素知之甚少。还有关于农场对当地人口中耐药性传播的影响的辩论。这是对53个奶牛场进行的为期两年的监测研究。E.大肠杆菌blaCTX-M阳性224/4145（5.4%）的粪便污染的网站。E. blaCTX-M阳性的大肠杆菌在小牛样品中更普遍（98/631; 15.5%），而在从牧场（包括公共场所）收集的样品中较不普遍（12/630; 1.9%）。多水平、多变量逻辑回归显示，抗生素干奶牛治疗选择与blaCTX-M阳性风险相关，包括使用头孢喹肟或新霉素B; 74%的blaCTX-M阳性E.大肠杆菌对新霉素耐药。低温与blaCTX-M阳性的低风险相关。这是温度对总E的影响的补充。大肠杆菌密度，这一发现对监测具有深远的意义。没有证据表明研究农场对当地人群中的循环blaCTX-M质粒有显著影响：在296个完全测序的E.大肠杆菌分离株，两个牛分离株共享blaCTX-M质粒与8个尿液分离株平行收集。
Little is known about the drivers of critically important antibacterial resistance in species with zoonotic potential present on farms (e.g. CTX-M β-lactamase-positive Escherichia coli). There is also debate about the influence of farms on the circulation of resistance in local human populations. This was a two-year surveillance study on 53 dairy farms. E. coli positive for blaCTX-M were detected in 224/4145 (5.4%) of all samples from faecally-contaminated sites. E. coli positive for blaCTX-M were more prevalent (98/631; 15.5%) in calf samples and less prevalent (12/630; 1.9%) in samples collected from pastureland, including publicly accessible sites. Multilevel, multivariable logistic regression showed antibiotic dry cow therapeutic choice to be associated with risk of blaCTX-M positivity, including use of cefquinome or framycetin; 74% of blaCTX-M-positive E. coli were framycetin-resistant. Low temperature was associated with low risk of blaCTX-M positivity. This was additional to the effect of temperature on total E. coli density, a finding with profound implications for surveillance. There was no evidence that study farms had a significant impact on circulating blaCTX-M plasmids in the local human population: across 296 fully sequenced E. coli isolates, two cattle isolates shared blaCTX-M plasmids with eight urinary isolates collected in parallel.
影响因子：
3.5
作者：
Pereira RV;Siler JD;Ng JC;Davis MA;Grohn YT;Warnick LD
通讯作者：
Warnick LD