A role for Q/N-rich aggregation-prone regions in P-body localization.
A role for Q/N-rich aggregation-prone regions in P-body localization.
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DOI：
10.1242/jcs.024976
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发表时间：
2008-08-01
影响因子：
4
通讯作者：
Beggs JD
中科院分区：
文献类型：
--
作者：
Reijns MA;Alexander RD;Spiller MP;Beggs JD
P体是细胞质病灶，是mRNA降解和翻译抑制的位点。目前尚不清楚是什么原因导致P体中RNA降解因子的积累，尽管RNA是必需的。酵母Lsm 1 - 7 p复合物在某些应激条件下被募集到P体。它是mRNA有效去帽和降解所必需的，但不是P体组装所必需的。在这里，我们表明，Lsm 4p亚基和它的天冬酰胺丰富的羧基末端容易聚集，这种聚集的趋势促进有效的积累Lsm 1 - 7 p的P-机构。Q/N-丰富的地区在其他P-体组件的存在下，建议一个更普遍的作用，在P-体的本地化和组装的聚集倾向的残基。这是由Ccr 4p，Pop 2 p和Dhh 1 p的P-体积累减少这些结构域删除后，并通过观察到的Q/N-丰富的区域从Ccr 4p的聚集支持。
P-bodies are cytoplasmic foci that are sites of mRNA degradation and translational repression. It is not known what causes the accumulation of RNA degradation factors in P-bodies, although RNA is required. The yeast Lsm1-7p complex is recruited to P-bodies under certain stress conditions. It is required for efficient decapping and degradation of mRNAs, but not for the assembly of P-bodies. Here we show that the Lsm4p subunit and its asparagine-rich carboxy-terminus are prone to aggregation and that this tendency to aggregate promotes efficient accumulation of Lsm1-7p in P-bodies. The presence of Q/N-rich regions in other P-body components suggests a more general role for aggregation-prone residues in P-body localization and assembly. This is supported by reduced P-body accumulation of Ccr4p, Pop2p and Dhh1p after deletion of these domains, and by the observed aggregation of the Q/N-rich region from Ccr4p.
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影响因子：
21.3
作者：
Jakymiw, A;Lian, SL;Chan, EKL
通讯作者：
Chan, EKL
影响因子：
4
作者：
Jakymiw, Andrew;Pauley, Kaleb M.;Chan, Edward K. L.
通讯作者：
Chan, Edward K. L.
影响因子：
16
作者：
Mitchell, P;Tollervey, D
通讯作者：
Tollervey, D
影响因子：
7.5
作者：
Paushkin, S;Gubitz, AK;Dreyfuss, G
通讯作者：
Dreyfuss, G
影响因子：
11.4
作者：
Achsel, T;Brahms, H;Lührmann, R
通讯作者：
Lührmann, R