Optimising a method for aragonite precipitation in simulated biogenic calcification media.
Optimising a method for aragonite precipitation in simulated biogenic calcification media.
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DOI：
10.1371/journal.pone.0278627
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发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
Allison, Nicola
中科院分区：
文献类型：
--
作者：
Kellock, Celeste;Alvarez, Maria Cristina Castillo;Finch, Adrian;Penkman, Kirsty;Kroger, Roland;Clog, Matthieu;Allison, Nicola
解决诸如温度、pH值、生物分子和矿物生长速率等因素如何影响生物成因CaCO 3的地球化学和结构，对于有效开发古代理岩至关重要。在这里，我们优化了一种方法，从海水中沉淀碳酸钙多晶型文石，在严格控制的条件下，模拟饱和状态（Ω）的珊瑚钙化液。然后，我们使用的方法来探索的影响，天冬氨酸（珊瑚骨骼中最丰富的氨基酸之一）文石结构和形态。在330 mL海水体积中，使用≥200 mg文石晶种（表面积0.84 m2）为矿物生长提供表面，生成了Ω文石= 6.9-19.2的沉淀速率的可重现估计值。然而，未播种的降水在持续时间上变化很大，不能提供一致的降水率估计。低浓度的天冬氨酸（1-10 μM）促进文石形成，但高浓度（≥ 1 mM）抑制沉淀。体外沉淀的文石的拉曼光谱可以通过确保至少60%的分析的文石在体外沉淀（相当于使用200 mg的晶种并在体外沉淀300 mg文石）而与起始晶种的特征分离。天冬氨酸浓度≥ 1 mM时，文石的拉曼峰v1的半峰全宽显著增加，这反映了文石结构中旋转无序的增加。珊瑚骨骼中有机物含量的变化可以驱动拉曼文石ν1峰的FWHM的变化，并且如果不考虑，可能会混淆从该参数对钙化流体饱和状态的解释。
Resolving how factors such as temperature, pH, biomolecules and mineral growth rate influence the geochemistry and structure of biogenic CaCO3, is essential to the effective development of palaeoproxies. Here we optimise a method to precipitate the CaCO3 polymorph aragonite from seawater, under tightly controlled conditions that simulate the saturation state (Ω) of coral calcification fluids. We then use the method to explore the influence of aspartic acid (one of the most abundant amino acids in coral skeletons) on aragonite structure and morphology. Using ≥200 mg of aragonite seed (surface area 0.84 m2), to provide a surface for mineral growth, in a 330 mL seawater volume, generates reproducible estimates of precipitation rate over Ωaragonite = 6.9–19.2. However, unseeded precipitations are highly variable in duration and do not provide consistent estimates of precipitation rate. Low concentrations of aspartic acid (1–10 μM) promote aragonite formation, but high concentrations (≥ 1 mM) inhibit precipitation. The Raman spectra of aragonite precipitated in vitro can be separated from the signature of the starting seed by ensuring that at least 60% of the analysed aragonite is precipitated in vitro (equivalent to using a seed of 200 mg and precipitating 300 mg aragonite in vitro). Aspartic acid concentrations ≥ 1mM caused a significant increase in the full width half maxima of the Raman aragonite v1 peak, reflective of increased rotational disorder in the aragonite structure. Changes in the organic content of coral skeletons can drive variations in the FWHM of the Raman aragonite ν1 peak, and if not accounted for, may confuse the interpretation of calcification fluid saturation state from this parameter.
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