Antibodies for assessing circadian clock proteins in the rodent suprachiasmatic nucleus.
Antibodies for assessing circadian clock proteins in the rodent suprachiasmatic nucleus.
复制标题
DOI：
10.1371/journal.pone.0035938
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Weaver DR
中科院分区：
文献类型：
--
作者：
LeSauter J;Lambert CM;Robotham MR;Model Z;Silver R;Weaver DR
研究昼夜节律的机制以及大脑和生物钟对环境和生理挑战的反应需要评估生物钟蛋白的水平。然而，通常很难获得特异性且可靠地标记这些蛋白质的抗体。许多这些抗体还缺乏适当的验证。该项目的目标是生成并表征针对几种生物钟蛋白的抗体。我们检查了小鼠和仓鼠视交叉上核（SCN）时钟蛋白表达的高峰和低谷时间。此外，我们通过对相关基因进行靶向破坏的小鼠测试抗体来确认特异性。我们的结果确定了针对 PER1、PER2、BMAL1 和 CLOCK 的抗体，这些抗体可用于通过免疫细胞化学评估 SCN 中的生物钟蛋白。
Research on the mechanisms underlying circadian rhythmicity and the response of brain and body clocks to environmental and physiological challenges requires assessing levels of circadian clock proteins. Too often, however, it is difficult to acquire antibodies that specifically and reliably label these proteins. Many of these antibodies also lack appropriate validation. The goal of this project was to generate and characterize antibodies against several circadian clock proteins. We examined mice and hamsters at peak and trough times of clock protein expression in the suprachiasmatic nucleus (SCN). In addition, we confirmed specificity by testing the antibodies on mice with targeted disruption of the relevant genes. Our results identify antibodies against PER1, PER2, BMAL1 and CLOCK that are useful for assessing circadian clock proteins in the SCN by immunocytochemistry.
登录
查看更多内容
DOI：
10.1073/pnas.1107178108
发表时间：
2011-09-27
影响因子：
11.1
作者：
Lee, Hyeong-Min;Chen, Rongmin;Lee, Choogon
通讯作者：
Lee, Choogon
影响因子：
3.5
作者：
Beaule, Christian;Cheng, Hai-Ying M.
通讯作者：
Cheng, Hai-Ying M.
影响因子：
2.9
作者：
Ramanathan, C;Nunez, AA;Smale, L
通讯作者：
Smale, L
哺乳动物上颈部核中时钟基因激活的有序时空模式的表征。
DOI：
10.1111/j.1460-9568.2011.07682.x
发表时间：
2011-05
期刊：
The European journal of neuroscience
影响因子：
--
作者：
Foley NC;Tong TY;Foley D;Lesauter J;Welsh DK;Silver R
通讯作者：
Silver R
影响因子：
16.2
作者：
DeBruyne, Jason P.;Noton, Elizabeth;Reppert, Steven M.
通讯作者：
Reppert, Steven M.