An In Vitro Diagnostic for Multiple Sclerosis Based on C-peptide Binding to Erythrocytes.
An In Vitro Diagnostic for Multiple Sclerosis Based on C-peptide Binding to Erythrocytes.
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DOI：
10.1016/j.ebiom.2016.07.036
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发表时间：
2016-09
期刊：
影响因子：
11.1
通讯作者：
Spence, Dana M.
中科院分区：
文献类型：
--
作者：
Lockwood, Sarah Y.;Summers, Suzanne;Eggenberger, Eric;Spence, Dana M.
目的：探讨血液实验室检测在多发性硬化症(MS)患者识别中的作用。对多发性硬化症患者、非多发性硬化症神经系统疾病患者和健康对照组的全血进行离心法分离红细胞。加入外源性C-肽后，用酶联免疫吸附试验(ELISA)检测上清液中剩余的C-肽。队列包括患有多发性硬化症(n=386)、其他非多发性硬化症(OND)神经系统疾病(n=975)的受试者，以及健康对照组(n=39)。MS患者红细胞C-肽平均结合量(3.51±0.59)pmol显著高于非MS患者(2.23±0.51)pmol；P<0.001和健康对照组(1.99±0.32)pmol；P<0.001。以C肽摄取临界值3.04 pmoL为临界值，该试验的敏感性为98.3%，特异性为89.5%。根据灵敏度与1-选择性的比率生成的接收器-操作员特性(ROC)曲线导致曲线下的面积为0.97。外源性C-肽与红细胞结合在MS患者与非MS神经系统疾病和健康对照之间具有潜在的鉴别价值。本文报道一种多发性硬化症的血液诊断方法。基于外源性C-肽与采集的红细胞的结合，结果与年龄、病程、治疗方法无关。多发性硬化症(MS)缺乏生物标记物和护理点诊断，尽管在许多确诊患者中经常发现标志性特征。许多团体正在努力确定这一故障的原因。在这里，我们报告说，在通过简单的抽血获得的血液样本中添加一种在大多数人类中自然存在的分子，可以在疾病诊断阶段作为一种快速而简单的辅助测试。
To investigate the utility of a blood-based lab test as an aid in identifying patients with Multiple Sclerosis (MS). Whole blood from subjects with MS, non-MS neurologic diseases, and healthy controls was centrifuged to isolate erythrocytes. Following the addition of exogenous C-peptide, the supernatant was assayed for remaining C-peptide using an enzyme linked immunosorbent assay (ELISA). The cohort included subjects with MS (n = 86), other non-MS neurologic diseases (OND n = 75), and healthy controls (n = 39). The average C-peptide bound to erythrocytes in MS samples (3.51 ± 0.59 pmol) was significantly higher than non-MS subjects (2.23 ± 0.51 pmol; p < 0.001) and healthy controls (1.99 ± 0.32 pmol; p < 0.001). Using a cutoff of 3.04 pmol of C-peptide uptake, the test exhibited a sensitivity of 98.3% and specificity of 89.5%. A receiver-operator characteristic (ROC) curve generated from the ratio of the sensitivity to 1-selectivity resulted in an area under the curve of 0.97. Exogenous C-peptide binding to erythrocytes has potential value in distinguishing MS subjects from non-MS neurologic diseases and healthy controls. A blood-based diagnostic for Multiple Sclerosis is reported. Based on exogenous C-peptide binding to harvested red cells Results are independent of age, disease duration, therapies. Biomarkers and point of care diagnostics are lacking in Multiple Sclerosis (MS), despite hallmark features often found in many diagnosed patients. Efforts to determine causes of this breakdown are ongoing by many groups. Here, we report that the addition of a molecule that is naturally occurring in most humans to a sample of blood obtained in a simple blood draw, may serve as a fast and simple auxiliary test during the diagnosing stage of the disease.
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影响因子：
11.2
作者：
Polman CH;Reingold SC;Banwell B;Clanet M;Cohen JA;Filippi M;Fujihara K;Havrdova E;Hutchinson M;Kappos L;Lublin FD;Montalban X;O'Connor P;Sandberg-Wollheim M;Thompson AJ;Waubant E;Weinshenker B;Wolinsky JS
通讯作者：
Wolinsky JS
DOI：
10.1152/ajpregu.00811.2007
发表时间：
2008-03-01
期刊：
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY, INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
影响因子：
--
作者：
Nordquist, Lina;Lai, En Yin;Persson, A. Erik G.
通讯作者：
Persson, A. Erik G.
影响因子：
11.2
作者：
DOREDUFFY, P;CATALANOTTO, F;TESTA, MA
通讯作者：
TESTA, MA
DOI：
10.1097/shk.0000000000000127
发表时间：
2014-04
期刊：
Shock (Augusta, Ga.)
影响因子：
--
作者：
Slinko S;Piraino G;Hake PW;Ledford JR;O'Connor M;Lahni P;Solan PD;Wong HR;Zingarelli B
通讯作者：
Zingarelli B
C肽对微血管血流和血液流行病学的影响。
DOI：
10.1080/15438600490424532
发表时间：
2004-01
期刊：
EXPERIMENTAL DIABESITY RESEARCH
影响因子：
--
作者：
Forst, T;Kunt, T
通讯作者：
Kunt, T