Adaptive evolution and divergence of SERPINB3: a young duplicate in great Apes.
Adaptive evolution and divergence of SERPINB3: a young duplicate in great Apes.
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DOI：
10.1371/journal.pone.0104935
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Seixas S
中科院分区：
文献类型：
--
作者：
Gomes S;Marques PI;Matthiesen R;Seixas S
一系列的重复事件导致进化枝B丝氨酸蛋白酶抑制剂（SERPIN）的扩增，目前在脊椎动物中显示出大量的功能。因此，最近位于人18q21.3SERPIN簇中的重复SERPINB3和B4分别控制不同半胱氨酸和丝氨酸蛋白酶的活性。在这里，我们的目标是评估SERPINB3和B4与它们的靶蛋白酶的共同进化，以了解这些重复的加速发散的进化力量。灵长类序列的系统发育分析将复制事件定位于人科祖先（1030 Mya），并将SERPINB 3的出现定位于人科（109 Mya）。我们在整个SERPINB4/B3灵长类树和靶蛋白酶组织蛋白酶L2（CTSL 2）和G（CTSG）以及糜蛋白酶（CMA 1）中检测到强阳性选择的证据。在人亚科进化枝中，SERPINB3和组织蛋白酶S（CTSS）的适应性进化过程完全匹配，正选择作用下的位点大多位于抑制剂/蛋白酶界面。总之，我们的研究结果似乎有利于SERPINB3，CTSS和CTSL 2和SERPINB4和CTSG和CMA 1的共同进化假说。宿主-病原体相互作用驱动的加速进化的情况也是可能的，因为SERPINB3/B4是由感染因子释放的外源蛋白酶的有效抑制剂。最后，相似的表达模式和许多调控基序的共享表明新功能化是SERPINB3和B4重复的功能分歧的最佳拟合模型。
A series of duplication events led to an expansion of clade B Serine Protease Inhibitors (SERPIN), currently displaying a large repertoire of functions in vertebrates. Accordingly, the recent duplicates SERPINB3 and B4 located in human 18q21.3 SERPIN cluster control the activity of different cysteine and serine proteases, respectively. Here, we aim to assess SERPINB3 and B4 coevolution with their target proteases in order to understand the evolutionary forces shaping the accelerated divergence of these duplicates. Phylogenetic analysis of primate sequences placed the duplication event in a Hominoidae ancestor (∼30 Mya) and the emergence of SERPINB3 in Homininae (∼9 Mya). We detected evidence of strong positive selection throughout SERPINB4/B3 primate tree and target proteases, cathepsin L2 (CTSL2) and G (CTSG) and chymase (CMA1). Specifically, in the Homininae clade a perfect match was observed between the adaptive evolution of SERPINB3 and cathepsin S (CTSS) and most of sites under positive selection were located at the inhibitor/protease interface. Altogether our results seem to favour a coevolution hypothesis for SERPINB3, CTSS and CTSL2 and for SERPINB4 and CTSG and CMA1. A scenario of an accelerated evolution driven by host-pathogen interactions is also possible since SERPINB3/B4 are potent inhibitors of exogenous proteases, released by infectious agents. Finally, similar patterns of expression and the sharing of many regulatory motifs suggest neofunctionalization as the best fitted model of the functional divergence of SERPINB3 and B4 duplicates.
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DOI：
10.1073/pnas.97.1.67
发表时间：
2000-01-04
影响因子：
11.1
作者：
Gooptu, B;Hazes, B;Lomas, DA
通讯作者：
Lomas, DA
影响因子：
4
作者：
Bird, PI
通讯作者：
Bird, PI
影响因子：
2.9
作者：
Al-Khunaizi, M;Luke, CJ;Silverman, GA
通讯作者：
Silverman, GA
影响因子：
5.8
作者：
Arnold, K;Bordoli, L;Schwede, T
通讯作者：
Schwede, T
影响因子：
14.8
作者：
De Vries, Sjoerd J.;van Dijk, Marc;Bonvin, Alexandre M. J. J.
通讯作者：
Bonvin, Alexandre M. J. J.