Analysis of sodium 2-mercaptoethane sulfonate in rat plasma using high performance liquid chromatography tandem-mass spectrometry.
Analysis of sodium 2-mercaptoethane sulfonate in rat plasma using high performance liquid chromatography tandem-mass spectrometry.
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DOI：
10.1016/j.jchromb.2021.123088
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发表时间：
2022-01-15
期刊：
影响因子：
--
通讯作者：
Logue BA
中科院分区：
文献类型：
--
作者：
Donkor AB;White CW;Nick HJ;Logue BA
2-巯基乙烷磺酸钠（MESNA）是一种含硫醇的化合物，已证明其可有效灭活丙烯醛，丙烯醛是一些抗癌药物（例如，环磷酰胺和异环磷酰胺）。此外，它清除自由基，通过攻击生物分子引起许多疾病。目前可用于分析生物基质中MESNA的方法包括比色法和高效液相色谱法（HPLC）与紫外，荧光或电化学检测。这些方法有几个局限性，包括低灵敏度，选择性差，难度高，分析时间长。因此，开发并验证了一种快速、简单和灵敏的HPLC串联质谱（MS/MS）方法，用于定量IP给药后大鼠血浆中的MESNA。通过血浆蛋白沉淀、离心、上清液蒸发、复溶和HPLC-MS/MS分析完成MESNA分析。该方法具有出色的检测限（20 nM）、出色的线性（R2= 0.999，残留准确度百分比> 90%）和较宽的线性范围（0.05-200 μM）。该方法也产生了良好的准确度和精密度（分别为100±10%和<10%的相对标准偏差）。经验证的方法被成功地用于分析MESNA从治疗的动物，并将允许更容易地开发MESNA的治疗目的。
Sodium 2-mercaptoethane sulfonate (MESNA) is a thiol-containing compound that has proven to be effective in inactivating acrolein, the toxic metabolite of some anti-cancer drugs (e.g., cyclophosphamide and ifosphamide). Also, it scavenges free radicals which cause numerous disorders by attacking biological molecules. Current methods available to analyze MESNA in biological matrices include colorimetry and high-performance liquid chromatography (HPLC) with ultraviolet, fluorescence, or electrochemical detection. These methods have several limitations including low sensitivity, poor selectivity, a high degree of difficulty, and long analysis times. Hence, a rapid, simple, and sensitive HPLC tandem mass spectrometry (MS/MS) method was developed and validated to quantify MESNA in rat plasma following IP administration. The analysis of MESNA was accomplished via plasma protein precipitation, centrifugation, supernatant evaporation, reconstitution, and HPLC-MS/MS analysis. The method showcases an outstanding limit of detection (20 nM), excellent linearity (R2= 0.999, and percent residual accuracy > 90%) and a wide linear range (0.05–200 μM). The method also produced good accuracy and precision (100±10% and <10% relative standard deviation, respectively). The validated method was successfully used to analyze MESNA from treated animals and will allow easier development of MESNA for therapeutic purposes.
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影响因子：
2.9
作者：
ElYazigi, A;Ernst, P;Raines, DA
通讯作者：
Raines, DA
DOI：
10.1111/j.1365-2125.1987.tb03092.x
发表时间：
1987-05-01
影响因子：
3.4
作者：
JAMES, CA;MANT, TGK;ROGERS, HJ
通讯作者：
ROGERS, HJ
影响因子：
1.8
作者：
Mare, S;Penugonda, S;Ercal, N
通讯作者：
Ercal, N
影响因子：
3.9
作者：
ELLMAN, GL
通讯作者：
ELLMAN, GL
影响因子：
3.1
作者：
Haggag, Rim S.;Gawad, Dina A.;Elbardisy, Hadil M.
通讯作者：
Elbardisy, Hadil M.