The use of exfoliative cell samples to map clonal genetic alterations in the oral epithelium of high-risk patients.
The use of exfoliative cell samples to map clonal genetic alterations in the oral epithelium of high-risk patients.
复制标题
使用脱落细胞样本来绘制高危患者口腔上皮的克隆遗传改变图。
作者：
M. Rosin;Joel B. Epstein;Ken Berean;S. Durham;John B. Hay;Xing Cheng;Tao Zeng;Yongqian Huang;Lewei Zhang
尽管克隆遗传改变是肿瘤进展的重要组成部分这一观点已被广泛接受，但对这种改变在高危病变中的分布或这些克隆如何随时间发生改变知之甚少。我们探讨了使用刮粘膜表面收集脱落细胞检测22例患者（14例鳞状细胞癌，2例原位癌和6例发育不良）口腔病变中等位基因丢失的可行性。数据显示，在这些样品中观察到的等位基因丢失模式密切代表在相同区域的活检中观察到的那些。此外，早期迹象表明，这种方法可用于检测治疗后患者中改变细胞克隆的复发性生长。
Although it is widely accepted that clonal genetic alterations are an essential component of tumor progression, little is known of the distribution of such changes in high-risk lesions or how such clones are altered over time. We explored the feasibility of using exfoliative cells collected by scraping the mucosal surface to detect allelic loss in oral lesions of 22 patients (14 squamous cell carcinomas, 2 carcinomas in situ, and 6 dysplasias). The data show that the patterns of allelic loss observed in these samples closely represent those observed in biopsies of the same region. Furthermore, early indications are that this approach can be used to detect recurrent outgrowth of clones of altered cells in patients after therapy.
DOI：
10.1073/pnas.89.13.5847
发表时间：
1992-07-01
影响因子：
11.1
作者：
ZHANG, L;CUI, XF;ARNHEIM, N
通讯作者：
ARNHEIM, N
影响因子：
158.5
作者：
BRENNAN, JA;MAO, L;SIDRANSKY, D
通讯作者：
SIDRANSKY, D